https://scholars.lib.ntu.edu.tw/handle/123456789/390359
標題: | Establishment of a DsRed-monomer-harboring ICR transgenic mouse model and effects of the transgene on tissue development | 作者: | Chou, C.-J. Peng, S.-Y. CHO-HUA WAN Chen, S.-F. Cheng, W.T.K. Lin, K.-Y. SHINN-CHIH WU |
關鍵字: | DsRed-monomer; ICR; Red fluorescence protein; Transgenic mice | 公開日期: | 2015 | 卷: | 58 | 期: | 1 | 來源出版物: | Chinese Journal of Physiology | 摘要: | DsRed-monomer is an enhanced red fluorescent protein that may serve as a marker for studies in biotechnology and cell biology. Since the ICR mouse strain is a widely utilized outbred strain for oncology, toxicology, vaccine development and for aging studies, the objective of this study was to produce a DsRed-monomer transgenic mouse by means of pronuclear micro-injection of a vector driven by the cytomegalovirus (CMV) enhancer/chicken beta-actin promoter. Four transgenic mice were successfully produced, one of which expressed the DsRed-monomer protein in every tissue, although at varying levels. High expression levels were observed in the heart, pancreas and muscle. Moreover, amniotic fluid-derived progenitor cells, which also expressed the DsRed-monomer protein, could be collected from the DsRed-monomer- harboring ICR mice. As compared to wild-type mice, a few biochemical and histological dissimilarities were found in the DsRed-monomer transgenic mice, including the presence of intra-cytoplasmic eosinophilic threadlike materials in the acinar cells. Taken together, transgenic mice stably expressing DsRed-monomer can be produced using pronuclear micro-injection; however, expression of the DsRed-monomer gene or its insertion position may lead to minor influences. ? 2015 by The Chinese Physiological Society and Airiti Press Inc. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84937943747&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/390359 |
DOI: | 10.4077/CJP.2015.BAC264 | SDG/關鍵字: | red fluorescent protein; fluorescent dye; fluorescent protein 583; photoprotein; acinar cell; amniotic fluid stem cell; animal experiment; animal tissue; Article; cell differentiation; controlled study; gene expression; histology; microinjection; mouse; nonhuman; organogenesis; protein expression; protein localization; red fluorescent protein gene; transgene; transgenic mouse; wild type; animal; genetics; Institute for Cancer Research mouse; transgenic mouse; Animals; Fluorescent Dyes; Luminescent Proteins; Mice; Mice, Inbred ICR; Mice, Transgenic; Microinjections; Transgenes |
顯示於: | 動物科學技術學系 |
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