https://scholars.lib.ntu.edu.tw/handle/123456789/390381
標題: | Suppression of Th2 immune responses by the sulfated polysaccharide from Porphyra haitanensis in tropomyosin-sensitized mice | 作者: | TZU-MING PAN | 關鍵字: | Food allergy; Mouse model; Porphyra haitanensis; Sulfated polysaccharide; Th2 immune response; Tropomyosin | 公開日期: | 2015 | 卷: | 24 | 期: | 2 | 起(迄)頁: | 211-218 | 來源出版物: | International Immunopharmacology | 摘要: | The sulfated polysaccharide from Porphyrawas hypothesized to exhibit immunoregulatory, anti-tumor and antiinflammatory activity, but its anti-allergic activity is not fully understood. Therefore, the aimof this study was to isolate sulfated polysaccharide fromPorphyra haitanensis (PHPS) and investigate its anti-allergic potential using a tropomyosin (TM)-induced mouse allergy model. Intraperitoneal injection of PHPS suppressed the allergic reaction by modulating serum IgE, IgG1 and IgG2a levels in mice. In particular, when PHPS was injected prior to the first immunization with TM, the IgE level decreased by 34.2% compared with the control (PBS) group. Oral therapeutic administration of PHPS to TM-sensitized mice decreased histamine release and repaired the pathology in the jejunumof the small intestine. In vitro, the mRNA expressions of the TM-induced Th2 cytokines (interleukin- 4 (IL-4), IL-5 and IL-13) in splenic lymphocyteswere reduced by PHPS; however, the expression of Th1 and regulatory cytokines (interferon gamma (IFN-γ) and IL-10)were up-regulated inPHPS-treated splenic lymphocytes. In the splenic lymphocyte supernatant, the IL-4, IL-13 and IFN-γ levels were also regulated by PHPS. Moreover, PHPS induced IFN-γ secretion via the Jun N-terminal kinase (JNK) and Janus kinase 2 (JAK2) signaling pathways. Therefore, these results suggest that PHPS suppresses the TM-induced allergic reaction, possibly by modulating the imbalance of the Th1/Th2 immune response. ? 2014 Elsevier B.V. All rights reserved. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84919681321&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/390381 |
DOI: | 10.1016/j.intimp.2014.11.019 | SDG/關鍵字: | algal extract; antiallergic agent; galactose; gamma interferon; histamine; immunoglobulin E; immunoglobulin G1; immunoglobulin G2a; interleukin 10; interleukin 13; interleukin 4; interleukin 5; Janus kinase 2; messenger RNA; polysaccharide sulfate; stress activated protein kinase; tropomyosin; allergen; cytokine; immunoglobulin E; Janus kinase 2; mitogen activated protein kinase kinase 4; polysaccharide; sulfate; tropomyosin; animal cell; animal experiment; animal model; animal tissue; antiallergic activity; Article; cellular immunity; comparative study; controlled study; cytokine production; drug activity; drug isolation; drug purification; feces level; female; food allergy; histamine blood level; histamine release; histopathology; immune response; immunization; immunoblotting; immunoglobulin blood level; in vitro study; interferon production; mouse; nonhuman; Porphyra; Porphyra haitanensis; protein blood level; protein expression; protein secretion; signal transduction; spleen cell; Th1 cell; Th1 Th2 balance; Th2 cell; upregulation; Western blotting; animal; Bagg albino mouse; blood; cell culture; cellular immunity; chemistry; disease model; drug effects; human; humoral immunity; hypersensitivity; immunology; immunosuppressive treatment; metabolism; Porphyra; Th2 cell; Allergens; Animals; Cells, Cultured; Cytokines; Disease Models, Animal; Female; Humans; Hypersensitivity; Immunity, Cellular; Immunity, Humoral; Immunization; Immunoglobulin E; Immunosuppression; Janus Kinase 2; MAP Kinase Kinase 4; Mice, Inbred BALB C; Polysaccharides; Porphyra; Signal Transduction; Sulfates; Th1 Cells; Th2 Cells; Tropomyosin |
顯示於: | 生化科技學系 |
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