Time-dependent cellular response in the liver and heart in a dietary-induced obese mouse model: the potential role of ER stress and autophagy
Journal
European Journal of Nutrition
Journal Volume
55
Journal Issue
6
Pages
2031
Date Issued
2016-09-01
Author(s)
Abstract
Purpose: Both endoplasmic reticulum stress (ER stress) and autophagy are essential for the response of the protein quality control system to cellular stresses. This study investigated the influence of the duration of a high-fat diet (HFD) in mice on tissue-specific cellular responses, specifically with regard to the role of autophagy and ER stress. Methods: Male mice aged 6–7 weeks were fed ad libitum with a standard chow diet or with a HFD for 2, 4, 8, or 16 weeks. Results: The HFD progressively increased mean body weight and induced tissue hypertrophy. The expression of PERK was suppressed in the liver after 16 weeks of the HFD and in the heart after 8 weeks of the HFD. Procaspase 12 and its activated form were induced in the liver with the HFD after 2 weeks, but not in the heart over the 16-week period. The activation of hepatic AMPK was elevated following 4 weeks of the HFD, but was inhibited after 16 weeks of the HFD. The ratio of LC3II to LC3I in the liver did not increase except in those mice fed the HFD for 16 weeks. The expression of AMPK and LC3 in the heart did not change over the entire 16 weeks of feeding the HFD. Cleaved PARP was increased in the liver and heart of mice receiving the HFD for 8 weeks. Conclusions: This study provides evidence that a HFD affects the cellular protein quality control processes responsible for metabolic disorder in a tissue- and duration-dependent manner. © 2015, Springer-Verlag Berlin Heidelberg.
Subjects
Apoptosis; Autophagy; Endoplasmic reticulum stress; High-fat diet; Hypertrophy
SDGs
Other Subjects
caspase; caspase 12; cell protein; hydroxymethylglutaryl coenzyme A reductase kinase; initiation factor 2alpha; LC3I protein; LC3II protein; membrane protein; procaspase 12; protein kinase; protein kinase rna like endoplasmic reticulum kinase; unclassified drug; adenylate kinase; MAP1LC3 protein, mouse; microtubule associated protein; PERK kinase; protein kinase R; triacylglycerol; animal cell; animal experiment; animal model; animal tissue; Article; autophagy; body weight; cellular stress response; controlled study; diet induced obesity; endoplasmic reticulum stress; enzyme activation; enzyme activity; heart; hypertrophy; lipid diet; liver; male; mouse; nonhuman; protein expression; weight gain; adverse effects; animal; blood; C57BL mouse; cardiac muscle; cytology; disease model; Dyslipidemias; hyperglycemia; hypertrophy; liver; metabolism; mouse mutant; obesity; pathology; time factor; Adenylate Kinase; Animals; Autophagy; Diet, High-Fat; Disease Models, Animal; Dyslipidemias; eIF-2 Kinase; Endoplasmic Reticulum Stress; Hyperglycemia; Hypertrophy; Liver; Male; Mice; Mice, Inbred C57BL; Mice, Obese; Microtubule-Associated Proteins; Myocardium; Obesity; Time Factors; Triglycerides
Publisher
Dr. Dietrich Steinkopff Verlag GmbH and Co. KG
Type
journal article