https://scholars.lib.ntu.edu.tw/handle/123456789/391348
標題: | Development of therapeutic au-methylene blue nanoparticles for targeted photodynamic therapy of cervical cancer cells | 作者: | Yu, J. Hsu, C.-H. Huang, C.-C. Chang, P.-Y. JIASHING YU |
關鍵字: | gold@polymer; methylene blue; nanoparticles; photodynamic therapy; transferrin | 公開日期: | 2015 | 卷: | 7 | 期: | 1 | 起(迄)頁: | 432-441 | 來源出版物: | ACS Applied Materials and Interfaces | 摘要: | Photodynamic therapy (PDT) involves the cellular uptake of a photosensitizer (PS) combined with oxygen molecules and light at a specific wavelength to be able to trigger cancer cell death via the apoptosis pathway, which is less harmful and has less inflammatory side effect than necrosis. However, the traditional PDT treatment has two main deficiencies: the dark toxicity of the PS and the poor selectivity of the cellular uptake of PS between the target cells and normal tissues. In this work, methylene blue (MB), a known effective PS, combined with Au nanoparticles (NPs) was prepared using an intermolecular interaction between a polystyrene-alt-maleic acid (PSMA) layer on the Au NPs and MB. The Au@polymer/MB NPs produced a high quantum yield of singlet oxygen molecules, over 50% as much as that of free MB, when they were excited by a dark red light source at 660 nm, but without significant dark toxicity. Furthermore, transferrin (Tf) was conjugated on the Au@polymer/MB NPs via an EDC/NHS reaction to enhance the selectivity to HeLa cells compared to 3T3 fibroblasts. With a hand-held single laser treatment (32 mW/cm) for 4 min, the new Au@polymer/MB-Tf NPs showed a 2-fold enhancement of PDT efficiency toward HeLa cells over the use of free MB at 4 times dosage. Cellular staining examinations showed that the HeLa cells reacted with Au@polymer/MB-Tf NPs and the 660 nm light excitation triggered PDT, which caused the cells to undergo apoptosis ( programmed cell death). We propose that applying this therapeutic Au@polymer/MB-Tf nanoagent is facile and safe for delivery and cancer cell targeting to simultaneously minimize side effects and accomplish a significant enhancement in photodynamic therapeutic efficiency toward next-generation nanomedicine development. ? 2014 American Chemical Society. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84921287623&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/391348 |
DOI: | 10.1021/am5064298 | SDG/關鍵字: | Aromatic compounds; Cell culture; Cell death; Conjugated polymers; Cytology; Diseases; Functional polymers; Gold; Light sources; Medical nanotechnology; Molecules; Nanoparticles; Organic polymers; Oxygen; Photosensitizers; Polymers; Toxicity; Cancer cell targeting; Cervical cancer cells; Intermolecular interactions; Methylene Blue; Photodynamic therapy (PDT); Programmed cell deaths; Therapeutic efficiency; transferrin; Photodynamic therapy; gold; maleic acid; maleic acid derivative; metal nanoparticle; methylene blue; oxygen; photosensitizing agent; polystyrene derivative; reactive oxygen metabolite; transferrin; 3T3 cell line; animal; apoptosis; chemistry; computer assisted tomography; cytology; female; fibroblast; HeLa cell line; human; mouse; nanotechnology; optics; photochemotherapy; procedures; Uterine Cervical Neoplasms; 3T3 Cells; Animals; Apoptosis; Female; Fibroblasts; Gold; HeLa Cells; Humans; Maleates; Metal Nanoparticles; Methylene Blue; Mice; Nanotechnology; Optics and Photonics; Oxygen; Photochemotherapy; Photosensitizing Agents; Polystyrenes; Reactive Oxygen Species; Tomography, X-Ray Computed; Transferrin; Uterine Cervical Neoplasms |
顯示於: | 化學工程學系 |
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