Effects of Grifola frondosa non-polar bioactive components on high-fat diet fed and streptozotocin-induced hyperglycemic mice
Journal
Pharmaceutical Biology
Journal Volume
53
Journal Issue
5
Pages
705-709
Date Issued
2015
Author(s)
Abstract
Context: Consumption of medicinal mushrooms for disease prevention and maintaining health has a very long history in Asia. Grifola frondosa (Fr) S.F. Gray (GF) (Meripilaceae) is a medicinal fungus popularly used for enhancing immune systems, lowering blood glucose, and improving spleen, stomach, and nerve functions. Objective: This study examines the hypoglycemic effects of GF in vitro and in vivo, and analyzes the chemical profiles of its bioactive components. Materials and methods: In vitro hypoglycemic effects of GF was evaluated enzymatically using α-amylase and α-glucosidase inhibition assays, whereas in vivo study was conducted on high-fat diet fed and streptozotocin (HFD + STZ)-induced hyperglycemic mice. GC-MS was used to determine the chemical profiles of bioactive components. Results: The non-polar fraction of GF exhibited a stronger anti-α-glucosidase activity (IC50: 0.0332 mg/ml) than acarbose, but its anti-α-amylase activity (IC50: 0.671 mg/ml) was weaker. Oral administration of GF at 600 mg/kg (GF600) significantly lowered the blood glucose, HbA1c, average blood glucose, and serum total cholesterol levels in hyperglycemic mice. Although GF was found to contain mainly oleic acid and linoleic acid, their levels in the fungus were low, suggesting that the effects of GF on HFD + STZ-induced hyperglycemic mice could be due to factors other than these fatty acids. Conclusion: These results conclude that GF possesses anti-α-glucosidase activity, and hypoglycemic effect in HFD + STZ-induced hyperglycemic mice. ? 2014 Informa Healthcare USA, Inc. All rights reserved.
Subjects
Diabetes; Fatty acids; Hypoglycemic activity; Maitake; Medicinal fungus; Mushroom; α-Glucosidase
SDGs
Other Subjects
amylase; Grifola frondosa extract; hemoglobin A1c; heptadecanoic acid; linoleic acid; oleic acid; palmitic acid; palmitoleic acid; plant extract; stearic acid; unclassified drug; biological factor; streptozocin; animal experiment; animal model; animal tissue; antidiabetic activity; Article; cholesterol blood level; controlled study; enzyme inhibition assay; glucose blood level; Grifola frondosa; hemoglobin blood level; hyperglycemia; in vitro study; in vivo study; lipid diet; male; mass fragmentography; mouse; nonhuman; tissue homogenate; adverse effects; animal; blood; C57BL mouse; chemically induced; Grifola; hyperglycemia; isolation and purification; lipid diet; treatment outcome; Basidiomycota; Fungi; Grifola frondosa; Mus; Animals; Biological Factors; Diet, High-Fat; Grifola; Hyperglycemia; Male; Mice; Mice, Inbred C57BL; Streptozocin; Treatment Outcome
Type
journal article