https://scholars.lib.ntu.edu.tw/handle/123456789/391983
標題: | Tracking and Finding Slow-Proliferating/Quiescent Cancer Stem Cells with Fluorescent Nanodiamonds | 作者: | Lin, H.-H. Lee, H.-W. Lin, R.-J. Huang, C.-W. Liao, Y.-C. Chen, Y.-T. Fang, J.-M. Lee, T.-C. Yu, A.L. Chang, H.-C. YIT-TSONG CHEN JIM-MIN FANG |
關鍵字: | cancers; diamonds; fluorescence imaging; nanoparticles; stem cells | 公開日期: | 2015 | 卷: | 11 | 期: | 34 | 起(迄)頁: | 4394-4402 | 來源出版物: | Small | 摘要: | Quiescent cancer stem cells (CSCs) have long been considered to be a source of tumor initiation. However, identification and isolation of these cells have been hampered by the fact that commonly used fluorescent markers are not sufficiently stable, both chemically and photophysically, to allow tracking over an extended period of time. Here, it is shown that fluorescent nanodiamonds (FNDs) are well suited for this application. Genotoxicity tests of FNDs with comet and micronucleus assays for human fibroblasts and breast cancer cells indicate that the nanoparticles neither cause DNA damage nor impair cell growth. Using AS-B145-1R breast cancer cells as the model cell line for CSC, it is found that the FND labeling outperforms 5-ethynyl-2′-deoxyuridine (EdU) and carboxyfluorescein diacetate succinimidyl ester (CFSE) in regards to its long-term tracking capability (>20 d). Moreover, through a quantification of their stem cell activity by measuring mammosphere-forming efficiencies (MFEs) and self-renewal rates, the FND-positive cells are identified to have an MFE twice as high as that of the FND-negative cells isolated from the same dissociated mammospheres. Thus, the nanoparticle-based labeling technique provides an effective new tool for tracking and finding slow-proliferating/quiescent CSCs in cancer research. ? 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84941191100&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/391983 |
DOI: | 10.1002/smll.201500878 | SDG/關鍵字: | Cell culture; Cytology; Diamonds; Diseases; Fluorescence; Nanodiamonds; Nanoparticles; Stem cells; Breast cancer cells; Cancers; Carboxyfluorescein diacetate; Fluorescence imaging; Fluorescent markers; Labeling techniques; Long-term tracking; Succinimidyl ester; Cells; mutagenic agent; nanodiamond; cancer stem cell; cell cycle; cell proliferation; cell survival; cell tracking; chemistry; comet assay; confocal microscopy; cytology; drug effects; fibroblast; flow cytometry; fluorescence; human; multicellular spheroid; pathology; procedures; Cell Cycle; Cell Proliferation; Cell Survival; Cell Tracking; Comet Assay; Fibroblasts; Flow Cytometry; Fluorescence; Humans; Microscopy, Confocal; Mutagens; Nanodiamonds; Neoplastic Stem Cells; Spheroids, Cellular |
顯示於: | 化學系 |
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