Cloning and functional characterization of a monoterpene synthase gene from Eleutherococcus trifoliatus
Journal
Holzforschung
Journal Volume
69
Journal Issue
2
Pages
163-171
Date Issued
2015
Author(s)
Abstract
Abstract Eleutherococcus trifoliatus also known as the three-leaved Eleutherococcus , a member of the Araliaceae (ginseng) family, is commonly used in traditional Chinese medicine. Recently, many studies have demonstrated the bioactivities of the secondary metabolites in E. trifoliatus . In this study, a monoterpene synthase from E. trifoliatus has been characterized. A pair of degenerate primers was designed and a fragment with conserved region of terpene synthase (TPS) was obtained. After 5′- and 3′-rapid amplification of cDNA ends (RACE), the full-length cDNA was obtained. The gene designated EtLIM contains an open reading frame of 1752 bp with a predicated molecular mass of 67.3 kDa. It was expressed in young leaves, stems, and drupes. The product of EtLIM has been identified by gas chromatography/mass spectrometry (GC/MS) as limonene.
Type
journal article