https://scholars.lib.ntu.edu.tw/handle/123456789/396637
標題: | Relationship of bovine TNF-α gene polymorphisms with the risk of bovine tuberculosis in Holstein cattle | 作者: | Cheng, Y. Huang, C.S. HSIANG-JUNG TSAI |
關鍵字: | Bovine tuberculosis; Polymorphism; Susceptibility; TNF-α | 公開日期: | 2016 | 卷: | 78 | 期: | 5 | 起(迄)頁: | 727-732 | 來源出版物: | Journal of Veterinary Medical Science | 摘要: | Many studies suggest significant genetic variation in the resistance of cattle and humans to infection with Mycobacterium bovis (M. bovis), the causative agent of zoonotic tuberculosis. TNF-α promotes inflammation and induces apoptosis in response to mycobacterial infection. The aim of the present study was to investigate the influence of single nucleotide polymorphisms of the TNF-α gene on bovine tuberculosis (bTB) susceptibility. We genotyped the TNF-α gene in 74 bTB-infected Holstein cows and 90 healthy control animals. The influence in the exon 3 region of TNF-α polymorphisms on bTB susceptibility was subsequently investigated by association analysis. Our finding demonstrated that the g.27534932A>C polymorphism of the TNF-α is associated with bTB in Holstein cattle. The susceptibility of cattle with the g.27534932A>C genotype compared with the CC genotype was 4.11-fold (95% CI, 1.27–13.36; P=0.02) higher. The g.27534932A>C polymorphism located in exon 3 of the TNF-α gene, and the functional consequence was missense. The deduced amino acid sequence for the protein product revealed an arginine to serine conversion at position 159, which may affect initiation of protein synthesis and disrupt normal TNF-α function that protects animals against mycobacterial infection. A significant association was observed with the A allele as a risk factor for bTB susceptibility (OR, 3.84; 95% CI, 1.21–12.17; P=0.02). In conclusion, this is the first report showing that the g.27534932A>C polymorphism may contribute to TNF-α-mediated bTB susceptibility. ? 2016 The Japanese Society of Veterinary Science. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84971401710&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/396637 |
DOI: | 10.1292/jvms.15-0506 | SDG/關鍵字: | tumor necrosis factor; animal; bovine; bovine tuberculosis; case control study; female; genetic predisposition; genetics; male; missense mutation; physiology; risk factor; single nucleotide polymorphism; Animals; Case-Control Studies; Cattle; Female; Genetic Predisposition to Disease; Male; Mutation, Missense; Polymorphism, Single Nucleotide; Risk Factors; Tuberculosis, Bovine; Tumor Necrosis Factor-alpha |
顯示於: | 獸醫學系 |
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