|Title:||Development of a multiplex Luminex assay for detecting swine antibodies to structural and nonstructural proteins of foot-and-mouth disease virus in Taiwan||Authors:||Chen, T.-H.
|Keywords:||Antibody detection; Foot-and-mouth disease virus; Multiplex Luminex assay; Nonstructural protein; Structural protein||Issue Date:||2016||Journal Volume:||49||Journal Issue:||2||Start page/Pages:||196-207||Source:||Journal of Microbiology, Immunology and Infection||Abstract:||
Background/Purpose(s): Foot-and-mouth disease (FMD) and swine vesicular disease (SVD) are serious vesicular diseases that have devastated swine populations throughout the world. The aim of this study was to develop a multianalyte profiling (xMAP) Luminex assay for the differential detection of antibodies to the FMD virus of structural proteins (SP) and nonstructural proteins (NSP). Methods: After the xMAP was optimized, it detected antibodies to SP-VP1 and NSP-3ABC of the FMD virus in a single serum sample. These tests were also compared with 3ABC polypeptide blocking enzyme-linked immunosorbent assay (ELISA) and virus neutralization test (VNT) methods for the differential diagnosis and assessment of immune status, respectively. Results: To detect SP antibodies in 661 sera from infected na?ve pigs and vaccinated pigs, the diagnostic sensitivity (DSn) and diagnostic specificity (DSp) of the xMAP were 90.0-98.7% and 93.0-96.5%, respectively. To detect NSP antibodies, the DSn was 90% and the DSp ranged from 93.3% to 99.1%. The xMAP can detect the immune response to SP and NSP as early as 4 days postinfection and 8 days postinfection, respectively. Furthermore, the SP and NSP antibodies in all 15 vaccinated but unprotected pigs were detected by xMAP. A comparison of SP and NSP antibodies detected in the sera of the infected samples indicated that the results from the xMAP had a high positive correlation with results from the VNT and a 3ABC polypeptide blocking ELISA assay. However, simultaneous quantitation detected that xMAP had no relationship with the VNT. Furthermore, the specificity was 93.3-94.9% with 3ABC polypeptide blocking ELISA for the FMDV-NSP antibody. Conclusion: The results indicated that xMAP has the potential to detect antibodies to FMDV-SP-VP1 and NSP-3ABC and to distinguish FMDV-infected pigs from pigs infected with the swine vesicular disease virus. ? 2014.
|DOI:||10.1016/j.jmii.2014.05.009||metadata.dc.subject.other:||3ABC protein, virus; capsid protein; viral protein; virus antibody; virus antigen; VP1 protein, Foot-and-mouth disease virus; animal; blood; differential diagnosis; Enterovirus B; evaluation study; Foot and mouth disease virus; Foot-and-Mouth Disease; immunoassay; immunology; pig; procedures; sensitivity and specificity; Swine Diseases; Taiwan; Animals; Antibodies, Viral; Antigens, Viral; Capsid Proteins; Diagnosis, Differential; Enterovirus B, Human; Foot-and-Mouth Disease; Foot-and-Mouth Disease Virus; Immunoassay; Sensitivity and Specificity; Swine; Swine Diseases; Taiwan; Viral Nonstructural Proteins
|Appears in Collections:||獸醫學系|
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