https://scholars.lib.ntu.edu.tw/handle/123456789/396911
標題: | Arsenite exposure accelerates aging process regulated by the transcription factor DAF-16/FOXO in Caenorhabditis elegans | 作者: | Yu, C.-W. How, C.M. VIVIAN LIAO |
關鍵字: | Aging; Arsenic; Caenorhabditis elegans; DAF-16; ROS | 公開日期: | 2016 | 卷: | 150 | 起(迄)頁: | 632-638 | 來源出版物: | Chemosphere | 摘要: | Arsenic is a known human carcinogen and high levels of arsenic contamination in food, soils, water, and air are of toxicology concerns. Nowadays, arsenic is still a contaminant of emerging interest, yet the effects of arsenic on aging process have received little attention. In this study, we investigated the effects and the underlying mechanisms of chronic arsenite exposure on the aging process in Caenorhabditis elegans. The results showed that prolonged arsenite exposure caused significantly decreased lifespan compared to non-exposed ones. In addition, arsenite exposure (100 μM) caused significant changes of age-dependent biomarkers, including a decrease of defecation frequency, accumulations of intestinal lipofuscin and lipid peroxidation in an age-dependent manner in C. elegans. Further evidence revealed that intracellular reactive oxygen species (ROS) level was significantly increased in an age-dependent manner upon 100 μM arsenite exposure. Moreover, the mRNA levels of transcriptional makers of aging (hsp-16.1, hsp-16.49, and hsp-70) were increased in aged worms under arsenite exposure (100 μM). Finally, we showed that daf-16 mutant worms were more sensitive to arsenite exposure (100 μM) on lifespan and failed to induce the expression of its target gene sod-3 in aged daf-16 mutant under arsenite exposure (100 μM). Our study demonstrated that chronic arsenite exposure resulted in accelerated aging process in C. elegans. The overproduction of intracellular ROS and the transcription factor DAF-16/FOXO play roles in mediating the accelerated aging process by arsenite exposure in C. elegans. This study implicates a potential ecotoxicological and health risk of arsenic in the environment. ? 2016 Elsevier Ltd. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84961322378&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/396911 |
DOI: | 10.1016/j.chemosphere.2016.01.004 | SDG/關鍵字: | Aging of materials; Arsenic; Health risks; Transcription; Transcription factors; Accelerated aging; Arsenic contamination; Arsenite exposures; Caenorhabditis elegans; DAF-16; Ecotoxicological; Lipid peroxidation; Reactive oxygen species; Chemical contamination; arsenic trioxide; heat shock protein; lipofuscin; reactive oxygen metabolite; transcription factor DAF 16; transcription factor FOXO; arsenous acid derivative; Caenorhabditis elegans protein; daf-16 protein, C elegans; forkhead transcription factor; pollutant; age; arsenite; biomarker; carcinogen; ecotoxicology; gene expression; health risk; mutation; nematode; pollution effect; pollution exposure; aging; Article; Caenorhabditis elegans; controlled study; fungal strain; lipid peroxidation; nonhuman; oxidative stress; pollutant; protein expression; reverse transcription polymerase chain reaction; aging; animal; Caenorhabditis elegans; drug effects; genetics; growth, development and aging; human; metabolism; oxidation reduction reaction; toxicity; Caenorhabditis elegans; Aging; Animals; Arsenites; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Environmental Pollutants; Forkhead Transcription Factors; Humans; Lipid Peroxidation; Lipofuscin; Oxidation-Reduction; Oxidative Stress; Reactive Oxygen Species |
顯示於: | 生物環境系統工程學系 |
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