https://scholars.lib.ntu.edu.tw/handle/123456789/397323
標題: | Development of an In Planta system to monitor phosphorus status by agroinfiltration and agroinjection | 作者: | Lin, J.-Y. Chiou, T.-J. HSIAO-FENG LO SHU-I LIN |
關鍵字: | Agroinfiltration; Agroinjection; Phosphorus; Tobacco; Tomato | 公開日期: | 2016 | 卷: | 409 | 期: | 1-2 | 起(迄)頁: | 313-328 | 來源出版物: | Plant and Soil | 摘要: | Background and Aims: An optimal supply of phosphate (Pi) fertilizer is important to ensure crop yield and quality and to maintain agricultural sustainability. In this study, an in planta monitoring system was developed to conveniently monitor the P status of crops. Methods: A phosphate starvation-induced gene (TPSI1) from tomato (Solanum lycopersicum L.) was used to reflect P status in plants. Using agroinfiltration and agroinjection techniques, the GUS reporter gene driven by the TPSI1 promoter was transiently expressed in tobacco (Nicotiana benthamiana) leaves and tomato fruits to reflect their P status. Results: We showed that the vegetative growth of tobacco and the yield and quality of tomato were affected by the supply of Pi fertilizer. We further demonstrated that the expression of a GUS reporter gene driven by the TPSI1 promoter could accurately report on P status in tobacco leaves and tomato fruits in real time. Expression of the GUS reporter gene was independent of deficiencies in mineral nutrients other than P, demonstrating the specificity of this system. Conclusions: The above results indicate that the employed agroinfiltration/agroinjection-based diagnostic system is useful for monitoring P status in plants. © 2016, Springer International Publishing Switzerland. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84976350817&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/397323 |
DOI: | 10.1007/s11104-016-2959-1 | SDG/關鍵字: | alternative agriculture; crop plant; crop yield; fertilizer application; fruit; gene expression; growth rate; leaf area; monitoring system; nutritional status; phosphorus; tobacco; Lycopersicon esculentum; Nicotiana benthamiana; Nicotiana tabacum |
顯示於: | 園藝暨景觀學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。