https://scholars.lib.ntu.edu.tw/handle/123456789/397506
標題: | Production of live offspring from vitrified-warmed oocytes collected at metaphase i stage | 作者: | Chang, C.-C. Chang, W.-F. Xu, J. Cheng, A.-S. Chang, C.-C. Nagy, Z.P. Yang, C.-C. Ding, S.-T. LI-YING SUNG |
公開日期: | 2016 | 卷: | 11 | 期: | 6 | 來源出版物: | PLoS ONE | 摘要: | Vitrification of matured oocytes is widely adopted in human clinics and animal research laboratories. Cryopreservation of immature oocytes, particularly those at metaphase I (MI), remains a challenge. In the present work, mouse MI oocytes denuded of cumulus cells were vitrified and warmed (V/W) either prior to (V/W-BEFORE-IVM, n = 562) or after (V/W-AFTER-IVM, n = 664) in vitro maturation (IVM). Derivative metaphase II (MII) oocytes were then used for intracytoplasmic sperm injection (ICSI). In the control groups, in vivo matured MII oocytes were used freshly (FRESH-MII, n = 517) or after V/W (MII-V/W, n = 617). In vitro and in vivo developmental competencies were compared among groups. Satisfactory blastocyst rates were achieved in V/W-BEFORE-IVM (27.5%) and V/W-AFTER-IVM (32.4%) groups, albeit as expected still lower than those from fresh-MII (56.1%) or MII-V/W (45.6%) oocytes. Similarly, the term development rates from V/W-BEFORE-IVM and V/W-AFTER-IVM were 12.4% and 16.7% respectively, acceptable but lower than those of the fresh-MII (41.2%) and MII-V/W (23.3%) groups. These data demonstrate that oocytes collected at MI stage are amenable to V/W, which can be performed before or after IVM with acceptable development rates including production of healthy pups. These findings provide useful knowledge to researchers and clinical practitioners for preservation and use of the otherwise discarded MI oocytes. ? 2016 Chang et al. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84976531708&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/397506 |
DOI: | 10.1371/journal.pone.0157785 | SDG/關鍵字: | blastocyst; control group; cumulus cell; female; human; in vitro study; intracytoplasmic sperm injection; major clinical study; maturation; metaphase; oocyte; physician; progeny; scientist; animal; C57BL mouse; cell survival; cytology; embryo development; embryo transfer; in vitro oocyte maturation; male; newborn; oocyte; procedures; specimen handling; vitrification; Animals; Animals, Newborn; Cell Survival; Embryo Transfer; Embryonic Development; Female; In Vitro Oocyte Maturation Techniques; Male; Metaphase; Mice, Inbred C57BL; Oocytes; Specimen Handling; Sperm Injections, Intracytoplasmic; Vitrification |
顯示於: | 生物科技研究所 |
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