https://scholars.lib.ntu.edu.tw/handle/123456789/397607
標題: | Exosomal miR-10a derived from amniotic fluid stem cells preserves ovarian follicles after chemotherapy | 作者: | Xiao, Guan-Yu Cheng, Chun-Chun Chiang, Yih-Shien Cheng, Winston Teng-Kuei Liu, I-Hsuan I-HSUAN LIU Shinn-Chih Wu |
公開日期: | 2016 | 卷: | 6 | 來源出版物: | Scientific Reports | 摘要: | Chemotherapy (CTx)-induced premature ovarian failure (POF) in woman remains clinically irreversible. Amniotic fluid stem cells (AFSCs) have shown the potential to treat CTx-induced POF; however, the underlying mechanism is unclear. Here we demonstrate that AFSC-derived exosomes recapitulate the anti-apoptotic effect of AFSCs on CTx-damaged granulosa cells (GCs), which are vital for the growth of ovarian follicles. AFSC-derived exosomes prevent ovarian follicular atresia in CTx-treated mice via the delivery of microRNAs in which both miR-146a and miR-10a are highly enriched and their potential target genes are critical to apoptosis. The down-regulation of these two miRNAs in AFSC-derived exosomes attenuates the anti-apoptotic effect on CTx-damaged GCs in vitro. Further, the administration of these miRNAs recapitulates the effects both in vitro and in vivo, in which miR-10a contributes a dominant influence. Our findings illustrate that miR-10a has potential as a novel therapeutic agent for the treatment of POF. |
URI: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000372060800001&KeyUID=WOS:000372060800001 http://scholars.lib.ntu.edu.tw/handle/123456789/397607 |
DOI: | 10.1038/srep23120 | SDG/關鍵字: | alkylating agent; cyclophosphamide; microRNA; MIRN10 microRNA, mouse; Mirn146 microRNA, mouse; amnion fluid; animal; apoptosis; binding site; cell culture; cell survival; chemically induced; coculture; cytology; drug effects; exosome; female; gene silencing; Institute for Cancer Research mouse; nucleotide sequence; ovary follicle; ovary follicle atresia; physiology; Primary Ovarian Insufficiency; RNA interference; secretion (process); stem cell; stem cell transplantation; Amniotic Fluid; Animals; Antineoplastic Agents, Alkylating; Apoptosis; Base Sequence; Binding Sites; Cell Survival; Cells, Cultured; Coculture Techniques; Cyclophosphamide; Exosomes; Female; Follicular Atresia; Gene Knockdown Techniques; Mice, Inbred ICR; MicroRNAs; Ovarian Follicle; Primary Ovarian Insufficiency; RNA Interference; Stem Cell Transplantation; Stem Cells |
顯示於: | 動物科學技術學系 |
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