https://scholars.lib.ntu.edu.tw/handle/123456789/398378
標題: | Cell refractive index for cell biology and disease diagnosis: Past, present and future | 作者: | P. Y. Liu L. K. Chin W. Ser H. F. Chen C.-M. Hsieh C.-H. Lee KUNG-BIN SUNG T. C. Ayi P. H. Yap B. Liedberg K. Wang T. Bourouina Y. Leprince-Wang |
公開日期: | 二月-2016 | 卷: | 16 | 期: | 4 | 起(迄)頁: | 634-644 | 來源出版物: | Lab on a Chip | 摘要: | Cell refractive index is a key biophysical parameter, which has been extensively studied. It is correlated with other cell biophysical properties including mechanical, electrical and optical properties, and not only represents the intracellular mass and concentration of a cell, but also provides important insight for various biological models. Measurement techniques developed earlier only measure the effective refractive index of a cell or a cell suspension, providing only limited information on cell refractive index and hence hindering its in-depth analysis and correlation. Recently, the emergence of microfluidic, photonic and imaging technologies has enabled the manipulation of a single cell and the 3D refractive index of a single cell down to sub-micron resolution, providing powerful tools to study cells based on refractive index. In this review, we provide an overview of cell refractive index models and measurement techniques including microfluidic chip-based techniques for the last 50 years, present the applications and significance of cell refractive index in cell biology, hematology, and pathology, and discuss future research trends in the field, including 3D imaging methods, integration with microfluidics and potential applications in new and breakthrough research areas. ? 2016 The Royal Society of Chemistry. |
URI: | http://scholars.lib.ntu.edu.tw/handle/123456789/398378 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84958048685&doi=10.1039%2fc5lc01445j&partnerID=40&md5=027d02b1cb7b7be51a0f50fbfa6d79d7 |
ISSN: | 14730197 | DOI: | 10.1039/c5lc01445j | SDG/關鍵字: | anemia; apoptosis; cell death; cell density; cell differentiation; cell division; cell granule; cell growth; cell membrane; cell nucleus; cell pH; cell refractive index; cell shape; cell structure; chemotaxis; circulating tumor cell; cytology; cytoplasm; cytosol; diffraction; hematology; hemoglobin determination; lysosome; malaria; microfluidics; mitochondrion; mitosis; monocyte; nuclear division; nuclear size; nucleolus; pathology; priority journal; refraction index; Review; three dimensional imaging; animal; diseases; human; procedures; refractometry; Animals; Cell Biology; Disease; Hematology; Humans; Refractometry |
顯示於: | 生醫電子與資訊學研究所 |
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