https://scholars.lib.ntu.edu.tw/handle/123456789/399515
標題: | Pu-erh Tea Extract Attenuates Nicotine-Induced Foam Cell Formation in Primary Cultured Monocytes: An in Vitro Mechanistic Study | 作者: | Tu, S.-H. MIN-HSIUNG PAN et al. |
關鍵字: | atherosclerosis; foam cell; tea components; α9-nicotinic acetylcholine receptor | 公開日期: | 2016 | 卷: | 64 | 期: | 16 | 起(迄)頁: | 3186-3195 | 來源出版物: | Journal of Agricultural and Food Chemistry | 摘要: | In this study, the mechanisms by which pu-erh tea extract (PETE) attenuates nicotine-induced foam cell formation were investigated. Monocytes were purified from healthy individuals using commercial antibodies coated with magnetic beads. We found that the nicotine-induced (1-10 μM) expression of oxidized low-density lipoprotein receptors (ox-LDLRs) and α9-nAchRs in monocytes was significantly attenuated by 24 h of PETE (10 μg/mL; ?, p < 0.05) cotreatment. Nicotine (1 μM for 24 h) significantly induced the expression of the surface adhesion molecule ICAM-1 and the monocyte integrin adhesion molecule (CD11b) by human umbilical vein endothelial cells (HUVECs) and triggered monocytes to differentiate into macrophages via interactions with the endothelium. After treatment with nicotine (0.1-10 μM for 24 h), the HUVECs released chemotactic factors (IL-8) to attract monocytes into the tunica intima of the artery, and the monocytes then transformed into foam cells. We demonstrated that PETE treatment (>1 μg/mL for 24 h; ?, p < 0.05) significantly attenuates nicotine-induced (1 μM) monocyte migration toward HUVECs and foam cell formation. This study suggests that tea components effectively attenuate the initial step (foam cell formation) of nicotine-induced atherosclerosis in circulating monocytes. ? 2016 American Chemical Society. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84966929703&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/399515 |
DOI: | 10.1021/acs.jafc.6b00624 | SDG/關鍵字: | Adhesion; Cells; Cellular manufacturing; Cytology; Diseases; Endothelial cells; Molecules; Nicotine; Acetylcholine receptor; atherosclerosis; Chemotactic factors; Foam cells; Human umbilical vein endothelial cells; Mechanistic studies; Oxidized low-density lipoproteins; tea components; Body fluids; nicotine; nicotinic receptor; plant extract; tea; cell culture; chemistry; cytology; drug effects; foam cell; human; metabolism; monocyte; tea; Cells, Cultured; Foam Cells; Humans; Monocytes; Nicotine; Plant Extracts; Receptors, Nicotinic; Tea |
顯示於: | 食品科技研究所 |
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