Development of a new type of multifunctional fucoidan-based nanoparticles for anticancer drug delivery
Journal
Carbohydrate Polymers
Journal Volume
165
Pages
410-420
Date Issued
2017
Author(s)
Abstract
Fucoidan, a sulfated marine polysaccharide, has many potential biological functions, including anticancer activity. Recently, fucoidan has been reported to target P-selectin expressed on metastatic cancer cells. Increasing research attention has been devoted to the developments of fucoidan-based nanomedicine. However, the application of traditional chitosan/fucoidan nanoparticles in anticancer drug delivery may be limited due to the deprotonation of chitosan at a pH greater than 6.5. In this study, a mutli-stimuli-responsive nanoparticle self-assembled by fucoidan and a cationic polypeptide (protamine) was developed, and their pH-/enzyme-responsive properties were characterized by circular dichroism (CD) spectroscopy, dynamic light scattering (DLS), and zeta potential analysis. Enzymatic digestion and acidic intracellular microenvironment (pH 4.5–5.5) in cancer cells triggered the release of an anticancer drug (doxorubicin) from the nanoparticles. The protamine/fucoidan complex nanoparticles with P-selectin mediated endocytosis, charge conversion and stimuli-tunable release properties showed an improved inhibitory effect against a metastatic breast cancer cell line (MDA-MB-231). ? 2017 Elsevier Ltd
Subjects
Drug delivery; Fucoidan; Metastatic cancer cells; Nanoparticles; P-selectin; Polypeptide
SDGs
Other Subjects
Cell culture; Chitin; Chitosan; Circular dichroism spectroscopy; Cytology; Dichroism; Diseases; Drug delivery; Dynamic light scattering; Enzyme activity; Light scattering; Medical nanotechnology; Molecular biology; Nanoparticles; Polypeptides; Anti-cancer drug delivery; Anticancer activities; Biological functions; Complex nanoparticles; Fucoidans; Metastatic cancer cells; P-selectin; Zeta potential analysis; Cells; antineoplastic agent; doxorubicin; fucoidin; nanoparticle; polysaccharide; chemistry; drug delivery system; human; pH; tumor cell line; Antineoplastic Agents; Cell Line, Tumor; Doxorubicin; Drug Delivery Systems; Humans; Hydrogen-Ion Concentration; Nanoparticles; Polysaccharides
Type
journal article