https://scholars.lib.ntu.edu.tw/handle/123456789/402161
標題: | Surface-modified cellulose paper and its application in infectious disease diagnosis | 作者: | Chun-Hui Yang Chung-An Chen CHIEN-FU CHEN |
關鍵字: | Colorimetric assay; Paper-based analytical device; Surface chemistry; Tuberculosis diagnosis | 公開日期: | 七月-2018 | 卷: | 265 | 起(迄)頁: | 506--513 | 來源出版物: | Sensors and Actuators B: Chemical | 摘要: | A sensitive and stable cellulose paper platform modified with carboxymethyl cellulose (CMC) was investigated for use as a rapid and accurate immunoassay for tuberculosis. Absorbing CMC to the paper substrate enables it to act as a linker for immobilizing biomolecules in a hydrophilic environment to promote their functionality and stability. This CMC-modified cellulose material can also be crosslinked with 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride/N-Hydroxy-succinimide (EDC/NHS) to generate NHS ester groups on the paper surface that can be covalently conjugated with ligands or target analytes for highly specific binding. As a result, with this system we were able to achieve a detection limit of 0.03 ng/mL for tuberculin-purified protein derivative. In addition, multiple tests can be completed simultaneously in as little as 21 min. These results demonstrate how this surface-modified paper analytical device can provide sensitive and high-throughput on-site disease diagnosis for resource-limited settings. ? 2018 Elsevier B.V. |
URI: | http://scholars.lib.ntu.edu.tw/handle/123456789/402161 | DOI: | 10.1016/j.snb.2018.03.092 | SDG/關鍵字: | Analytic equipment; Cellulose; Chemical analysis; Crosslinking; Surface chemistry; Carbodiimide hydrochlorides; Carboxymethyl cellulose; Colorimetric assays; Hydrophilic environments; Infectious disease; Modified cellulose; Paper-based analytical devices; Tuberculosis diagnosis; Diagnosis; Carboxymethyl Cellulose; Cellulose; Chemical Analysis; Diagnosis; Ester Groups; Paper |
顯示於: | 應用力學研究所 |
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