https://scholars.lib.ntu.edu.tw/handle/123456789/402162
Title: | Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device | Authors: | Kai-Hong Guo Pei-Hsuan Chen Chieh Lin Chien-Fu Chen I-Ren Lee Yi-Chun Yeh CHIEN-FU CHEN |
Keywords: | cueR; Cupriavidus metallidurans; paper device; smartphone-based diagnostic system; whole-cell biosensor | Issue Date: | Mar-2018 | Source: | ACS Sensors | Abstract: | This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine. ? 2018 American Chemical Society. |
URI: | http://scholars.lib.ntu.edu.tw/handle/123456789/402162 | DOI: | 10.1021/acssensors.7b00931 | SDG/Keyword: | gold; ion; luminescent agent; photoprotein; red fluorescent protein; chemistry; Cupriavidus; genetic engineering; genetic procedures; genetics; human; paper; procedures; urine; Biosensing Techniques; Cupriavidus; Genetic Engineering; Gold; Humans; Ions; Luminescent Agents; Luminescent Proteins; Paper |
Appears in Collections: | 應用力學研究所 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.