https://scholars.lib.ntu.edu.tw/handle/123456789/402184
標題: | Lipid-Modified Graphene-Transistor Biosensor for Monitoring Amyloid-β Aggregation | 作者: | Kuo, Chia-Jung Chiang, Hsu-Cheng Tseng, Chi-Ang Chang, Chin-Fu Ulaganathan, Rajesh Kumar Ling, Tzu-Ting Chang, Yu-Jen Chen, Chiao-Chen Chen, Yun-Ru Chen, Yit-Tsong YIT-TSONG CHEN |
關鍵字: | amyloid-beta; biosensor; cholera toxin; field-effect transistor; ganglioside GM1; graphene; phospholipase D; supported lipid bilayer | 公開日期: | 2018 | 卷: | 10 | 期: | 15 | 起(迄)頁: | 12311-12316 | 來源出版物: | ACS Applied Materials & Interfaces | 摘要: | A graphene field-effect transistor (G-FET) with the spacious planar graphene surface can provide a large-area interface with cell membranes to serve as a platform for the study of cell membrane-related protein interactions. In this study, a G-FET device paved with a supported lipid bilayer (referred to as SLB/G-FET) was first used to monitor the catalytic hydrolysis of the SLB by phospholipase D. With excellent detection sensitivity, this G-FET was also modified with a ganglioside GM1-enriched SLB (GM1-SLB/G-FET) to detect cholera toxin B. Finally, the GM1-SLB/G-FET was employed to monitor amyloid-beta 40 (Aβ40) aggregation. In the early nucleation stage of Aβ40 aggregation, while no fluorescence was detectable with traditional thioflavin T (ThT) assay, the prominent electrical signals probed by GM1-SLB/G-FET demonstrate that the G-FET detection is more sensitive than the ThT assay. The comprehensive kinetic information during the Aβ40 aggregation could be collected with a GM1-SLB/G-FET, especially covering the kinetics involved in the early stage of Aβ40 aggregation. These experimental results suggest that SLB/G-FETs hold great potential as a powerful biomimetic sensor for versatile investigations of membrane-related protein functions and interaction kinetics. ? 2018 American Chemical Society. |
URI: | https://doi.org/10.1021/acsami.8b01917 http://scholars.lib.ntu.edu.tw/handle/123456789/402184 |
DOI: | 10.1021/acsami.8b01917 | SDG/關鍵字: | Biomimetics; Biosensors; Cytology; Diseases; Field effect transistors; Glycoproteins; Graphene; Kinetics; Lipid bilayers; Proteins; Amyloid betas; Cholera toxin; ganglioside GM1; Phospholipase D; Supported lipid bilayers; Graphene transistors; ganglioside GM1; graphite; cell membrane; genetic procedures; lipid bilayer; Biosensing Techniques; Cell Membrane; G(M1) Ganglioside; Graphite; Lipid Bilayers |
顯示於: | 化學系 |
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