https://scholars.lib.ntu.edu.tw/handle/123456789/402828
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Martin, T. | en_US |
dc.contributor.author | Lou, Y.-C. | en_US |
dc.contributor.author | Chou, C.-C. | en_US |
dc.contributor.author | Wei, S.-Y. | en_US |
dc.contributor.author | Sadotra, S. | en_US |
dc.contributor.author | Cho, C.-C. | en_US |
dc.contributor.author | Lin, M.-H. | en_US |
dc.contributor.author | Tai, J.-H. | en_US |
dc.contributor.author | CHUN-HUA HSU | en_US |
dc.contributor.author | Chen, C. | en_US |
dc.creator | Martin, T.;Lou, Y.-C.;Chou, C.-C.;Wei, S.-Y.;Sadotra, S.;Cho, C.-C.;Lin, M.-H.;Tai, J.-H.;Hsu, C.-H.;Chen, C. | - |
dc.date.accessioned | 2018-09-10T18:07:11Z | - |
dc.date.available | 2018-09-10T18:07:11Z | - |
dc.date.issued | 2018 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-85044940577&partnerID=MN8TOARS | - |
dc.identifier.uri | http://scholars.lib.ntu.edu.tw/handle/123456789/402828 | - |
dc.description.abstract | Cyclophilin 1 (TvCyP1), a cyclophilin type peptidyl-prolyl isomerase present in the human parasite Trichomonas vaginalis, interacts with Myb1 and assists in its nuclear translocation. Myb1 regulates the expression of ap65-1 gene that encodes for a disease causing cytoadherence enzyme. Here, we determined the crystal structures of TvCyP1 and its complex with the minimum TvCyP1-binding sequence of Myb1 (Myb1104-111), where TvCyP1 formed a homodimer, unlike other single domain cyclophilins. In the complex structure, one Myb1104-111 peptide was bound to each TvCyP1 protomer, with G106-P107 and Y105 fitting well into the active site and auxiliary S2 pocket, respectively. NMR data further showed that TvCyP1 can catalyze the cis/trans isomerization of P107 in Myb1104-111. Interestingly, in the well-folded Myb1 protein (Myb135-141), the minimum binding sequence adopted a different conformation from that of unstructured Myb1104-111 peptide, that could make P107 binding to the active site of TvCyP1 difficult. However, NMR studies showed that similar to Myb1104-111 peptide, Myb135-141 also interacted with the active site of TvCyP1 and the dynamics of the Myb135-141 residues near P107 was reduced upon interaction. Together, the structure of TvCyP1 and detailed structural insights on TvCyP1-Myb1 interaction provided here could pave the way for newer drugs to treat drug-resistant strains. ? 2018 The Author(s). | - |
dc.language | en | en |
dc.relation.ispartof | Scientific Reports | en_US |
dc.source | AH-Scopus - Elsevier | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | cyclophilin; peptidylprolyl isomerase; protein binding; protozoal protein; transcription factor; binding site; chemistry; metabolism; molecular model; protein domain; protein multimerization; protein quaternary structure; protein stability; Trichomonas vaginalis; Binding Sites; Cyclophilins; Models, Molecular; Peptidylprolyl Isomerase; Protein Binding; Protein Domains; Protein Multimerization; Protein Stability; Protein Structure, Quaternary; Protozoan Proteins; Transcription Factors; Trichomonas vaginalis | - |
dc.title | Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis | - |
dc.type | journal article | en |
dc.identifier.doi | 10.1038/s41598-018-23821-5 | - |
dc.identifier.scopus | 2-s2.0-85044940577 | - |
dc.identifier.isi | WOS:000428994100003 | - |
dc.relation.journalvolume | 8 | - |
dc.relation.journalissue | 1 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Agricultural Chemistry | - |
crisitem.author.dept | Biochemical Sciences | - |
crisitem.author.dept | Genome and Systems Biology Degree Program | - |
crisitem.author.dept | Master Program in Global Agriculture Technology and Genomic Science (Global ATGS) | - |
crisitem.author.orcid | 0000-0002-0008-7383 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | International College | - |
顯示於: | 農業化學系 |
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