|Title:||Organic anion-transporting polypeptide 1B3 as a dual reporter gene for fluorescence and magnetic resonance imaging||Authors:||HON-MAN LIU||Issue Date:||2018||Journal Volume:||32||Journal Issue:||3||Start page/Pages:||1705-1715||Source:||FASEB Journal||Abstract:||
Reporter proteins have broad applications in visualizing molecular events at the cellular, tissue and whole-body levels. Transmembrane transporters recognizing specificmolecular domains are of particular interest because they enable the migration of signal-source molecules from the extracellular space to the cytoplasm for subsequent application in multimodality imaging. Organic anion-transporting polypeptides (OATPs) have demonstrated their MRI reporter efficacy. We further expanded their use as a dual-modality reporter in MRI and noninvasive in vivo imaging system (IVIS). We overexpressed OATP1B3 in the HT-1080 sarcoma cell line. Both Gd-EOB-DTPA, anMRI contrast agent, and indocyanine green (ICG), a near-infrared fluorescent dye that provides better deep-tissue detection because of its longwavelength, couldbe deliveredto the intracellular space and imaged in a tumor-bearing nudemousemodel. Our in vivo dual-imaging reporter systemachieved high sensitivity inMRI and observation periods lasting as long as 96 h in IVIS. Because of the superior temporal and spatial resolutions and the clinical availability of both ICG and Gd-EOB-DTPA, this dual-imaging OATP1B3 systemwill find biomedical use in tumor biology, stemcell trafficking, and tissue engineering. ? The Author(s).
|Appears in Collections:||醫學系|
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