https://scholars.lib.ntu.edu.tw/handle/123456789/403586
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | PO-LING KUO | en_US |
dc.contributor.author | Ching-Che Charng | en_US |
dc.contributor.author | Po-Chen Wu | en_US |
dc.contributor.author | PAI-CHI LI | en_US |
dc.creator | Po-Chen Wu;Ching-Che Charng;PAI-CHI LI;PO-LING KUO | - |
dc.date.accessioned | 2019-02-19T02:36:59Z | - |
dc.date.available | 2019-02-19T02:36:59Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 00219533 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/403586 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85010216152&doi=10.1242%2fjcs.186320&partnerID=40&md5=f6eb0598e84fadd78dbd51390d7b8f0c | - |
dc.description.abstract | Studying mechanobiology in three-dimensional (3D) cell cultures better recapitulates cell behaviors in response to various types of mechanical stimuli in vivo Stiffening of the extracellular matrix resulting from cell remodeling potentiates many pathological conditions, including advanced cancers. However, an effective tool for measuring the spatiotemporal changes in elastic properties of such 3D cell cultures without directly contacting the samples has not been reported previously. We describe an ultrasonic shear-wave-based platform for quantitatively evaluating the spatiotemporal dynamics of the elasticity of a matrix remodeled by cells cultured in 3D environments. We used this approach to measure the elasticity changes of 3D matrices grown with highly invasive lung cancer cells and cardiac myoblasts, and to delineate the principal mechanism underlying the stiffening of matrices remodeled by these cells. The described approach can be a useful tool in fields investigating and manipulating the mechanotransduction of cells in 3D contexts, and also has potential as a drug-screening platform. | en_US |
dc.language.iso | en | en_US |
dc.publisher | The Company of Biologists | en_US |
dc.relation.ispartof | Journal of Cell Science | en_US |
dc.subject | Matrix elasticity; Matrix remodeling; Three-dimensional; Animals; Anisotropy; Biophysics; Cell Culture Techniques; Cell Line, Tumor; Collagen; Extracellular Matrix; Humans; Hydrogel, Polyethylene Glycol Dimethacrylate; Myoblasts; Myocardium; Rats; Rheology; Spatio-Temporal Analysis; Temperature; Elasticity; Mechanotransduction, Cellular; Shear Strength | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | collagen gel; collagen; hydrogel; anisotropy; Article; cardiac myoblast; cell culture; controlled study; elasticity; extracellular matrix; flow measurement; human; human cell; in vitro study; lung cancer cell line; mechanotransduction; molecular dynamics; priority journal; shear flow; shear wave elastography; spatiotemporal analysis; three dimensional cell culture; ultrasound transducer; viscoelasticity; animal; biophysics; cardiac muscle; cell culture technique; cytology; drug effects; extracellular matrix; flow kinetics; hydrogel; metabolism; myoblast; pharmacology; procedures; rat; shear strength; temperature; tumor cell line; Animals; Anisotropy; Biophysics; Cell Culture Techniques; Cell Line, Tumor; Collagen; Elasticity; Extracellular Matrix; Humans; Hydrogel; Mechanotransduction, Cellular; Myoblasts; Myocardium; Rats; Rheology; Shear Strength; Spatio-Temporal Analysis; Temperature | - |
dc.title | Shear-wave elasticity measurements of three-dimensional cell cultures for mechanobiology | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1242/jcs.186320 | - |
dc.identifier.pmid | 27505887 | - |
dc.identifier.scopus | 2-s2.0-85010216152 | - |
dc.identifier.isi | WOS:000391105300030 | - |
dc.identifier.url | https://doi.org/10.1242/jcs.186320 | - |
dc.relation.pages | 292-302 | - |
dc.relation.journalvolume | 130 | - |
dc.relation.journalissue | 1 | - |
dc.identifier.external | 25903271 | - |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Biomedical Electronics and Bioinformatics | - |
crisitem.author.dept | Electrical Engineering | - |
crisitem.author.dept | Biomedical Electronics and Bioinformatics | - |
crisitem.author.dept | Electrical Engineering | - |
crisitem.author.orcid | 0000-0001-7603-7852 | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
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