https://scholars.lib.ntu.edu.tw/handle/123456789/404782
標題: | Chromosome-level assembly, genetic and physical mapping of Phalaenopsis aphrodite genome provides new insights into species adaptation and resources for orchid breeding | 作者: | Ya-Ting Chao Wan-Chieh Chen Chun-Yi Chen Hsiu-Yin Ho Chih-Hsin Yeh Yi-Tzu Kuo Chun-Lin Su Shao-Hua Yen Hao-Yen Hsueh Jen-Hau Yeh Hui-Lan Hsu Yi-Hui Tsai Tzu-Yen Kuo Song-Bin Chang Kai-Yi Chen |
關鍵字: | plant genome;orchid;de novo assembly;genetic mapping;restriction site-associated DNA sequencing;fluorescence in situ hybridization | 公開日期: | 2018 | 卷: | 16 | 起(迄)頁: | 2027-2041 | 來源出版物: | Plant Biotechnology Journal | 摘要: | The Orchidaceae is a diverse and ecologically important plant family. Approximately 69% of all orchid species are epiphytes, which provide diverse microhabitats for many small animals and fungi in the canopy of tropical rainforests. Moreover, many orchids are of economic importance as food flavourings or ornamental plants. Phalaenopsis aphrodite, an epiphytic orchid, is a major breeding parent of many commercial orchid hybrids. We provide a high-quality chromosomescale assembly of the P. aphrodite genome. The total length of all scaffolds is 1025.1 Mb, with N50 scaffold size of 19.7 Mb. A total of 28 902 protein-coding genes were identified. We constructed an orchid genetic linkage map, and then anchored and ordered the genomic scaffolds along the linkage groups. We also established a high-resolution pachytene karyotype of P. aphrodite and completed the assignment of linkage groups to the 19 chromosomes using fluorescence in situ hybridization. We identified an expansion in the epiphytic orchid lineage of FRS5-like subclade associated with adaptations to the life in the canopy. Phylogenetic analysis further provides new insights into the orchid lineage-specific duplications of MADS-box genes, which might have contributed to the variation in labellum and pollinium morphology and its accessory structure. To our knowledge, this is the first orchid genome to be integrated with a SNP-based genetic linkage map and validated by physical mapping. The genome and genetic map not only offer unprecedented resources for increasing breeding efficiency in horticultural orchids but also provide an important foundation for future studies in adaptation genomics of epiphytes. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/404782 | DOI: | 10.1111/pbi.12936 |
顯示於: | 農藝學系 |
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