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  1. NTU Scholars
  2. 醫學院
  3. 腫瘤醫學研究所
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/405039
Title: GALNT6 expression enhances aggressive phenotypes of ovarian cancer cells by regulating EGFR activity
Authors: Lin, Tzu-Chi
Chen, Syue-Ting
MIN-CHUAN HUANG
Huang, John
Hsu, Chia Lang
HSUEH-FEN JUAN 
HO-HSIUNG LIN
CHI-HAU CHEN
Keywords: GALNT6; O-glycosylation; epidermal growth factor receptor; invasion; ovarian cancer
Issue Date: 2017
Publisher: Impact Journals, LLC
Journal Volume: 8
Journal Issue: 26
Start page/Pages: 42588-42601
Source: Oncotarget 
Abstract: 
Ovarian cancer is the most lethal of the gynecologic malignancies. N-acetylgalactosaminyltransferase 6 (GALNT6), an enzyme that mediates the initial step of mucin type-O glycosylation, has been reported to regulate mammary carcinogenesis. However, the expression and role of GALNT6 in ovarian cancer are still unclear. Here we showed that high GALNT6 expression correlates with increased recurrence, lymph node metastasis, and chemoresistance in ovarian endometrioid and clear cell carcinomas; and higher GALNT6 levels are significantly associated with poorer patient survivals. GALNT6 knockdown with two independent siRNAs significantly suppressed viability, migration, and invasion of ovarian cancer cells. Using phospho-RTK array and Western blot analyses, we identified EGFR as a critical target of GALNT6. GALNT6 knockdown decreased phosphorylation of EGFR, whereas GALNT6 overexpression increased the phosphorylation. Lectin pull-down assays with Vicia villosa agglutinin (VVA) indicated that GALNT6 was able to modify O-glycans on EGFR. Moreover, the GALNT6-enhanced invasive behavior was significantly reversed by erlotinib, an EGFR inhibitor. Our results suggest that GALNT6 expression is associated with poor prognosis of ovarian cancer and enhances the aggressive behavior of ovarian cancer cells by regulating EGFR activity.
URI: https://scholars.lib.ntu.edu.tw/handle/123456789/405039
ISSN: 1949-2553
DOI: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000405493400069&KeyUID=WOS:000405493400069
10.18632/oncotarget.16585
metadata.dc.subject.other: [SDGs]SDG3
epidermal growth factor receptor; erlotinib; n acetylgalactosaminyltransferase; n acetylgalactosaminyltransferase 6; unclassified drug; adult; Article; cancer prognosis; cancer resistance; cell invasion; cell migration; cell viability; clear cell carcinoma; controlled study; disease free survival; endometrioid carcinoma; female; gene expression; human; human tissue; lymph node metastasis; major clinical study; ovarian cancer cell line; ovary carcinoma; overall survival; protein glycosylation; protein phosphorylation; tumor invasion; tumor recurrence
Appears in Collections:腫瘤醫學研究所

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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

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開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

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