|Title:||Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin||Authors:||SZU-HUA PAN
|Keywords:||Extracellular matrix; Hair follicle; Neogenesis; Protein factor; Regeneration; Reprogram||Issue Date:||Jun-2018||Publisher:||ELSEVIER SCI LTD||Journal Volume:||167||Start page/Pages:||121-131||Source:||Biomaterials||Abstract:||
Organ development is a sophisticated process of self-organization. However, despite growing understanding of the developmental mechanisms, little is known about how to reactivate them postnatally for regeneration. We found that treatment of adult non-hair fibroblasts with cell-free extract from embryonic skin conferred upon them the competency to regenerate hair follicles. Proteomics analysis identified three secreted proteins enriched in the embryonic skin, apolipoprotein-A1, galectin-1 and lumican that together were essential and sufficient to induce new hair follicles. These 3 proteins show a stage-specific co-enrichment in the perifolliculogenetic embryonic dermis. Mechanistically, exposure to embryonic skin extract or to the combination of the 3 proteins altered the gene expression to an inductive hair follicle dermal papilla fibroblast-like profile and activated Igf and Wnt signaling, which are crucial for the regeneration process. Therefore, a cocktail of organ-specific extracellular proteins from the embryonic environment can render adult cells competent to re-engage in developmental interactions for organ neogenesis. Identification of factors that recreate the extracellular context of respective developing tissues can become an important strategy to promote regeneration in adult organs.
|Appears in Collections:||醫學系|
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