|Title:||R26R-GR: a Cre-activable dual fluorescent protein reporter mouse||Authors:||YOU-TZUNG CHEN
Ku, Amy Tsu
Behringer, Richard R.
Nicolis, Silvia K.
|Issue Date:||2012||Publisher:||PUBLIC LIBRARY SCIENCE||Journal Volume:||7||Journal Issue:||9||Source:||PloS one||Abstract:||
Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent protein (mCherry). This dual labeling allows the visualization of mitotic events, cell shapes and intracellular vesicle behaviors. We expect this reporter mouse to have a wide application in developmental biology studies, transplantation experiments as well as cancer/stem cell lineage tracing.
|Appears in Collections:||基因體暨蛋白體醫學研究所|
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