https://scholars.lib.ntu.edu.tw/handle/123456789/406744
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Liu K.-N. | en_US |
dc.contributor.author | Wang H.-Y. | en_US |
dc.contributor.author | Chen C.-Y. | en_US |
dc.contributor.author | Wang S.S.-S. | en_US |
dc.creator | Chen C.-Y.;Wang H.-Y.;Liu K.-N.;Wang S.S.-S. | - |
dc.date.accessioned | 2019-05-09T04:08:33Z | - |
dc.date.available | 2019-05-09T04:08:33Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 09394451 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/406744 | - |
dc.description.abstract | This work examines the effects of L-arginine (L-Arg) on the aggregation and amyloid fibrillation of bovine serum albumin (BSA). We demonstrate that L-Arg dose-dependently reduces thioflavin T (ThT) fluorescence of BSA within the L-Arg concentration range used (0-1.4 M). However, as revealed by electron microscopy, size exclusion chromatography, and dynamic light scattering results, L-Arg does not prevent amyloid-like fibril formation by BSA. We conclude that L-Arg competes against ThT for binding sites on BSA amyloid-like fibrils, leading to biased results in ThT fluorescence measurements. Moreover, the use of ThT fluorescence assay to screen for potential inhibitors against amyloid fibrillation can give misleading results. ? Springer-Verlag 2010. | - |
dc.language | en | - |
dc.relation.ispartof | Amino Acids | en_US |
dc.subject | Aggregate | - |
dc.subject | Amyloid fibril | - |
dc.subject | Arginine | - |
dc.subject | Bovine serum album | - |
dc.subject | ThT fluorescence | - |
dc.title | L-Arginine reduces thioflavin T fluorescence but not fibrillation of bovine serum album | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1007/s00726-010-0536-0 | - |
dc.identifier.scopus | 2-s2.0-77956917818 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956917818&doi=10.1007%2fs00726-010-0536-0&partnerID=40&md5=6b5ae7a9af66a90f0bf7ad72c3fb044d | - |
dc.relation.pages | 821-829 | - |
dc.relation.journalvolume | 39 | - |
dc.relation.journalissue | 3 | - |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Chemical Engineering | - |
crisitem.author.orcid | exist | - |
crisitem.author.parentorg | College of Engineering | - |
顯示於: | 化學工程學系 |
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