https://scholars.lib.ntu.edu.tw/handle/123456789/406746
標題: | Amyloid fibrillation and cytotoxicity of insulin are inhibited by the amphiphilic surfactants | 作者: | Wang S.S.-S. Liu K.-N. Han T.-C. |
關鍵字: | Amyloid fibril;Cytotoxicity;Inhibition;Insulin;Phospholipid;Surfactant | 公開日期: | 2010 | 卷: | 1802 | 期: | 6 | 起(迄)頁: | 519-530 | 來源出版物: | Biochimica et Biophysica Acta - Molecular Basis of Disease | 摘要: | Amyloid fibrils have been associated with at least 25 different degenerative diseases. The 51-residue polypeptide hormone insulin, which is associated with type II diabetes, has been shown to self-assemble to form amyloid fibrils in vitro. With bovine insulin as a model, the research presented here explores the effects of two amphiphilic surfactants (1,2-dihexanoyl-sn-glycero-3-phosphocholine (di-C7-PC) and 1,2-diheptanoyl-sn-glycero-3-phosphocholine (di-C7-PC)) on the in vitro fibrillation process of bovine insulin at pH 2.0 and 55. ¢XC. We demonstrated that insulin fibrillation may be inhibited by both surfactants in a dose-dependent fashion. The best inhibition of fibril formation is observed when insulin is incubated with 4. mM di-C7-PC. Moreover, the addition of either surfactant at the concentrations studied attenuated insulin fibril-induced cytotoxicity in both PC12 and SH-SY5Y cell lines. The results from this work may contribute to the understanding of the molecular factors affecting amyloid fibrillation and the molecular mechanism(s) of the interactions between the membrane and amyloid proteins. ? 2010 Elsevier B.V. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/406746 | ISSN: | 09254439 | DOI: | 10.1016/j.bbadis.2010.02.008 | SDG/關鍵字: | 1,2 diheptanoyl sn glycero 3 phosphocholine; 1,2 dihexanoyl sn glycero 3 phosphocholine; amyloid; bovine insulin; glycerophosphorylcholine; unclassified drug; animal cell; article; beta sheet; cell culture; cell death; cell viability; circular dichroism; concentration response; controlled study; cytotoxicity; drug mechanism; fluorescence analysis; hormone action; human; human cell; in vitro study; non insulin dependent diabetes mellitus; nonhuman; priority journal; protein aggregation; protein secondary structure; rat; transmission electron microscopy; Amyloid; Amyloidosis; Animals; Cattle; Cell Line; Cell Survival; Humans; Insulin; Microscopy, Electron, Transmission; Models, Biological; PC12 Cells; Phosphatidylcholines; Phospholipid Ethers; Protein Multimerization; Protein Structure, Secondary; Rats; Surface-Active Agents; Bovinae |
顯示於: | 化學工程學系 |
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