https://scholars.lib.ntu.edu.tw/handle/123456789/407709
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Cheng C.-C. | en_US |
dc.contributor.author | Lee D.-J. | en_US |
dc.contributor.author | Chen J.-K. | en_US |
dc.creator | Chen J.-K.;Cheng C.-C.;Lee D.-J. | - |
dc.date.accessioned | 2019-05-13T09:35:44Z | - |
dc.date.available | 2019-05-13T09:35:44Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 17427061 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/407709 | - |
dc.description.abstract | Self-assembled supramolecular scaffolds, a combination of noncovalent interactions within a biocompatible polymer substrate, can be used for efficient construction of highly-controlled self-organizing hierarchical structures; these newly-developed biomaterials exhibit excellent mechanical properties, tunable surface hydrophilicity, low cytotoxicity and high biodegradability, making them highly attractive for tissue engineering and regenerative medicine applications. Herein, we demonstrate a novel supramolecular poly(£`-caprolactone) (PCL) containing self-complementary sextuple hydrogen-bonded uracil-diamidopyridine (U-DPy) moieties, which undergoes spontaneous self-assembly to form supramolecular polymer networks. Inclusion of various U-DPy contents enhanced the mechanical strength and viscosities of the resulting materials by up to two orders of magnitude compared to control PCL. Surface wettability and morphological studies confirmed physically-crosslinked films can be readily tailored to provide the desired surface properties. Cell viability assays indicated the excellent in vitro biocompatibility of U-DPy-functionalized substrates and indicate the potential of these materials for various biomedical applications. More importantly, mouse fibroblast NIH/3T3 cells cultured on these substrates displayed a more elongated cell morphology and had substantially higher cell densities than cells seeded on control PCL substrate, which indicates that introduction of U-DPy moieties into polymer matrixes could be used to create tissue culture surfaces that enhance cell attachment and proliferation. This new system is suggested as a potential route towards the practical realization of next-generation tissue-engineering scaffolds. Statement of Significance In this study, we report a significant breakthrough in development of self-assembled supramolecular polymers to form well-defined scaffolds through self-complementary hydrogen-bonding interactions. These newly developed materials exhibited extremely good mechanical properties, fine-tunable hydrophilic characteristics and excellent biocompatibility due to hydrogen-bond-induced physical cross-linking. Importantly, cell adhesion and proliferation assays indicated that these substrates efficiently promoted the growth of mouse embryonic fibroblasts NIH/3T3 cells in vitro. Thus, this finding provides a simple and effective route for the development of next-generation tissue-engineering scaffolds that have improved mechanical properties, increased surface hydrophilicity and can enhance the growth and biological activity of adherent cells. ? 2016 Acta Materialia Inc. | - |
dc.language | en | - |
dc.relation.ispartof | Acta Biomaterialia | en_US |
dc.subject | Bioactive scaffolds | - |
dc.subject | Multiple hydrogen bonds | - |
dc.subject | Self-assembly | - |
dc.subject | Supramolecular chemistry | - |
dc.subject | Tissue engineering | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Bioactivity; Biocompatibility; Biodegradability; Biomechanics; Cell adhesion; Cell culture; Cell engineering; Crosslinking; Fibroblasts; Hydrophilicity; Ions; Mammals; Medical applications; Scaffolds (biology); Self assembly; Substrates; Supramolecular chemistry; Tissue; Bioactive scaffold; Cell attachments; In-vitro; Multiple hydrogen bond; NIH-3T3 cells; Supramolecular polymers; Surface hydrophilicity; Tissue engineering scaffold; Tissues engineerings; Tunables; Hydrogen bonds; polycaprolactone; cross linking reagent; polycaprolactone; polyester; polymer; pyridine derivative; uracil; water; 3T3 cell line; animal experiment; Article; biocompatibility; cell adhesion; cell density; cell proliferation; cell structure; cell viability assay; controlled study; crystal structure; differential scanning calorimetry; hydrogen bond; hydrophilicity; in vitro study; mouse; nonhuman; priority journal; supramolecular chemistry; surface property; tissue engineering; tissue scaffold; viscosity; wettability; animal; cell adhesion; cell proliferation; cell survival; chemistry; cytology; fibroblast; flow kinetics; human; NIH 3T3 cell line; small angle scattering; tumor cell line; X ray diffraction; Animals; Cell Adhesion; Cell Line, Tumor; Cell Proliferation; Cell Survival; Cross-Linking Reagents; Fibroblasts; Humans; Hydrogen Bonding; Mice; NIH 3T3 Cells; Polyesters; Polymers; Pyridines; Rheology; Scattering, Small Angle; Uracil; Water; X-Ray Diffraction | - |
dc.title | Self-assembled supramolecular polymers with tailorable properties that enhance cell attachment and proliferation | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.actbio.2016.12.031 | - |
dc.identifier.scopus | 2-s2.0-85009275015 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009275015&doi=10.1016%2fj.actbio.2016.12.031&partnerID=40&md5=d6bf56d7a8dfc69ceb5945ced14d5cca | - |
dc.relation.pages | 476-483 | - |
dc.relation.journalvolume | 50 | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.orcid | 0000-0002-8820-8097 | - |
顯示於: | 化學工程學系 |
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