https://scholars.lib.ntu.edu.tw/handle/123456789/408656
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Nien P.-C. | en_US |
dc.contributor.author | Huang M.-C. | en_US |
dc.contributor.author | Chang F.-Y. | en_US |
dc.contributor.author | Ho K.-C. | en_US |
dc.creator | Chang F.-Y.;Huang M.-C.;Nien P.-C.;Ho K.-C. | - |
dc.date.accessioned | 2019-05-17T00:50:12Z | - |
dc.date.available | 2019-05-17T00:50:12Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 10400397 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/408656 | - |
dc.description.abstract | In this study, the flow injection analysis was applied to the enzyme-entrapped electrode on a chip for sensing glucose. The on-chip microelectrode was fabricated by the standard photolithography in clean-room environment and the microfluidic channel height of 100 mm on the chip was formed by poly(dimethylsiloxane). The conducting polymer, poly(3,4- ethylenedioxythiophene), PEDOT, was electropolymerized to entrap the coexisting glucose oxidase (GOD) by cyclic voltammetry (CV). The amount of enzyme entrapped in the matrix measured spectroscopically was about 0.101 U/cm2. At a flow rate of 10 ml/hr, the working electrode (Pt/PEDOT/GOD, WE1) was set at 0.7 V (vs. Ag/AgCl) and sensing of H2O2 was carried out by injecting samples with various concentrations of glucose (Glu). A linear relationship between the sensing current and the glucose concentration, ranging from 1 to 20 mM, was obtained with a sensitivity of 8 nA mm-2 mM -1. The response time and the recovery time were about 30 and 230 s, respectively. For a single-potential test, the oxidation currents of 0.08 mM ascorbic acid (AA) and a blend of 0.08 mM AA and 10 mM Glu reached 31.3% and 145.5%, respectively, when compared with the oxidation current of 10 mM Glu alone. However, when a pre-reactor (WE2) was set at the same potential (0.7 V) before the main enzyme integrated electrode (WE1), the oxidation current for the above mixed solution reached 99.6% of the original one. ? 2008 Wiley-VCH Verlag GmbH & Co. KGaA. | - |
dc.language | English | - |
dc.relation.ispartof | Electroanalysis | en_US |
dc.subject | Activity measurement | - |
dc.subject | Biosensor | - |
dc.subject | Enzyme | - |
dc.subject | Glucose | - |
dc.subject | Interference | - |
dc.subject | Microfluidic system | - |
dc.subject | On-chip microelectrode | - |
dc.title | Integrating an enzyme-entrapped conducting polymer electrode and a prereactor in a microfluidic system for sensing glucose | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1002/elan.200704123 | - |
dc.identifier.scopus | 2-s2.0-54549124614 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-54549124614&doi=10.1002%2felan.200704123&partnerID=40&md5=6491c29573f9b7f6bc31fab432072216 | - |
dc.relation.pages | 635-642 | - |
dc.relation.journalvolume | 20 | - |
dc.relation.journalissue | 6 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Chemical Engineering | - |
crisitem.author.dept | Polymer Science and Engineering | - |
crisitem.author.orcid | 0000-0001-7501-1271 | - |
crisitem.author.parentorg | College of Engineering | - |
crisitem.author.parentorg | College of Engineering | - |
顯示於: | 化學工程學系 |
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