|Title:||Synergistically Controlled Stemness and Multilineage Differentiation Capacity of Stem Cells on Multifunctional Biointerfaces||Authors:||Wu C.-Y.
|Keywords:||multifunctional biointerface;multilineage differentiation;stem cell spheroid;stemness;surface modification||Issue Date:||2017||Journal Volume:||4||Journal Issue:||11||Source:||Advanced Materials Interfaces||Abstract:||
Demonstrations of defined and controlled stem cell culture or differentiation advance fundamental life science research and the possibility of curing diseases. Here, the multifunctional concept of using defined matrix/interface modifications to support cell culture is demonstrated to provide synergistic coupling of concurrently immobilized fibroblast growth factor 2 and chitosan, and the resulting cell culture matrix/interface enables the proliferation of spheroids of adipose stem cells with enhanced stemness and the capacity to (trans-)differentiate into multiple cell lineages in the mesoderm, endoderm, and ectoderm. The modification and the coimmobilization are performed using a straightforward one-step vapor-based coating technology applicable to a wide range of cell culture materials. The coating technology raises the important prospect of designing a material interface that incorporates effective and sustainable factors to determine stem cell fate through the use of a facile multicomponent modification approach on culture substrates. ? 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
|Appears in Collections:||化學工程學系|
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