|Title:||Curcumin Induces Apoptosis of Colorectal Cancer Stem Cells by Coupling with CD44 Marker||Authors:||Huang Y.-T.
|Keywords:||apoptosis;cancer stem cells;CD44;colorectal cancer;colorectal cancer stem cells;curcumin||Issue Date:||2016||Journal Volume:||64||Journal Issue:||11||Start page/Pages:||2247-2253||Source:||Journal of Agricultural and Food Chemistry||Abstract:||
This study investigated the effect of curcumin on colorectal cancer stem cells (CCSCs) and its possible mechanism. Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5¡¬-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine. Our cell culture model study found that curcumin treatment (50 £gM for 48 h) did indeed increase apoptosis of CRC cells as well as of CCSCs, but at a significant level only in CD44+ cells. Further metabolic profile studies of the CRC, CD44+, and CD44- cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44- cells but decreased glutamine content in both curcumin-treated CRC and CD44+ cells. Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44+ coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44+ cells and inducing apoptosis. ? 2016 American Chemical Society.
|Appears in Collections:||食品科技研究所|
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