https://scholars.lib.ntu.edu.tw/handle/123456789/413297
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Kestwal R.M. | en_US |
dc.contributor.author | Bagal-Kestwal D. | en_US |
dc.contributor.author | Chiang B.H. | en_US |
dc.creator | Chiang B.H.;Bagal-Kestwal D.;Kestwal R.M. | - |
dc.date.accessioned | 2019-07-11T02:14:54Z | - |
dc.date.available | 2019-07-11T02:14:54Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 01418130 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/413297 | - |
dc.description.abstract | Endo-1,3(4)-£]-glucanase (EC 3.2.1.6) from Vigna aconitifolia sprouts was purified to 14.5 fold by gel filtration and ion-exchange chromatography. The enzyme was found to be a glycoprotein, its activity was Ca 2+ dependent and specific for £]-1,3 linkages in different polysaccharides. The K m value of the enzyme was estimated to be 3.0mgml -1 for £]-d-glucan as substrate. Circular dichroism studies revealed 8% £\-helix, 48% £]-pleated and 44% random coil in its secondary structure. Purified £]-glucanase was then successfully co-immobilized with glucose oxidase in agarose-chitosan beads, showing better immobilization yield, operational range and stability as compared with the crude £]-glucanase beads. The immobilized £]-glucanase was successfully used for mini-bioreactor fabrication. ? 2011 Elsevier B.V. | - |
dc.language | English | - |
dc.relation.ispartof | International Journal of Biological Macromolecules | - |
dc.subject | £]-Glucanase | - |
dc.subject | Characterization | - |
dc.subject | Immobilization | - |
dc.subject | Purification | - |
dc.subject | Vigna aconitifolia | - |
dc.title | 1,3-£]-Glucanase from Vigna aconitifolia and its possible use in enzyme bioreactor fabrication | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.ijbiomac.2011.08.002 | - |
dc.identifier.scopus | 2-s2.0-80054848383 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-80054848383&doi=10.1016%2fj.ijbiomac.2011.08.002&partnerID=40&md5=fa938abd81aeebe28b94ecd95759d7b6 | - |
dc.relation.pages | 894-899 | - |
dc.relation.journalvolume | 49 | - |
dc.relation.journalissue | 5 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Food Science and Technology | - |
crisitem.author.orcid | 0000-0002-0573-5650 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
顯示於: | 食品科技研究所 |
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