https://scholars.lib.ntu.edu.tw/handle/123456789/413527
Title: | Anti-depressant effects of Gastrodia elata Blume and its compounds gastrodin and 4-hydroxybenzyl alcohol, via the monoaminergic system and neuronal cytoskeletal remodeling | Authors: | Chen W.-C. Lai Y.-S. Lin S.-H. Lu K.-H. Lin Y.-E. Panyod S. Ho C.-T. Sheen L.-Y. |
Keywords: | 4-hydroxybenzylalcohol;Anti-depressant;Forcedswimmingtest;Gastrodia elataBlume;Gastrodin | Issue Date: | 2016 | Journal Volume: | 182 | Start page/Pages: | 190-199 | Source: | Journal of Ethnopharmacology | Abstract: | Ethnopharmacology relevance Gastrodia elata Blume is a highly valuable traditional Chinese medicine used in the treatment of depression. However, compounds with antidepressant effects in water extracts of G. elata Bl. (WGE) have not been identified. The aims of this study were to determine the major antidepressant compound in WGE and to evaluate the antidepressant effects of WGE and its active compounds which involved the monoaminergic system and neuronal cytoskeletal remodeling. Materials and methods Gastrodin (GAS) and 4-hydroxybenzyl alcohol (HBA) in WGE, were analyzed with high-performance liquid chromatography (HPLC)-ultraviolet detection. The forced swimming test (FST) was used to induce depression-like symptoms in 9 weeks old male Sprague-Dawley rats. The open field test (OFT) was used to measure anxiety after WGE, GAS, and HBA treatments. The levels of monoamine such as serotonin (5-HT), dopamine (DA), and their metabolites 5-hydroxyindoleacetic acid (5-HIAA), 3,4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) were measured using HPLC-electrochemical detection. Western blotting was used to examine the 5-HT1A receptor and the neuronal cytoskeleton remodeling-related proteins, Slit, dihydropyrimidinase-related protein 2 (DPYSL2, also called CRMP2), Ras homologous member A (RhoA), and profilin 1 (PFN1) in vivo. Slit1 expression was evaluated in Hs683 cell line after treated with WGE (0.5 mg/mL), GAS (50, 100 and 100 £gM), and HBA (50, 100 and 100 £gM). Results Oral administration of WGE (500 mg/kg bw), GAS (100 mg/kg bw), and HBA (100 mg/kg bw) exhibited the anti-depressant effect by significantly reducing the immobility time in FST, monoamine metabolism including the 5-HT to 5-HIAA in the hippocampus and DA to DOPAC and HVA ratios in the frontal cortex, amygdala, and hippocampus. In the hippocampus, the expression of the neuronal cytoskeleton remodeling-related negative regulators Slit1 and RhoA were significantly down-regulated. In addition, the positive regulators CRMP2 and PFN1 were significantly up-regulated following GAS, HBA, and WGE treatments. Moreover, WGE, GAS, and HBA were directly down-regulated Slit1 expression in Hs683 cells. Conclusion WGE, GAS, and HBA exhibited potential anti-depressant effects in rats by decreasing monoamine metabolism and modulated cytoskeleton remodeling-related protein expression in the Slit-Robo pathway. These results suggest that WGE can be used as agent for depressive prevention. ? 2016 Elsevier Ireland Ltd. All rights reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413527 | ISSN: | 3788741 | DOI: | 10.1016/j.jep.2016.02.001 | metadata.dc.subject.other: | [SDGs]SDG3 3,4 dihydroxyphenylacetic acid; 4 hydroxybenzyl alcohol; 5 hydroxyindoleacetic acid; alcohol derivative; antidepressant agent; collapsin response mediator protein 2; dopamine; Gastrodia elata extract; gastrodin; homovanillic acid; protein; RhoA guanine nucleotide binding protein; serotonin; Slit1 protein; Slit2 protein; unclassified drug; 3,4 dihydroxyphenylacetic acid; 4-hydroxybenzyl alcohol; 5 hydroxyindoleacetic acid; antidepressant agent; benzyl alcohol derivative; dopamine; gastrodin; glucoside; homovanillic acid; plant extract; serotonin; serotonin 1A receptor; animal behavior; animal experiment; animal model; antidepressant activity; Article; body weight; cancer cell; controlled study; depression; down regulation; forced swim test; frontal cortex; Gastrodia elata; high performance liquid chromatography; human; human cell; in vivo study; locomotion; male; monoamine metabolism; monoaminergic system; nerve cell plasticity; neuronal cytoskeletal remodeling; nonhuman; oligodendroglioma; open field test; protein expression; quantitative analysis; rat; upregulation; animal; brain; cytoskeleton; drug effects; exploratory behavior; Gastrodia; metabolism; nerve cell; Sprague Dawley rat; tumor cell line; 3,4-Dihydroxyphenylacetic Acid; Animals; Antidepressive Agents; Behavior, Animal; Benzyl Alcohols; Brain; Cell Line, Tumor; Cytoskeleton; Dopamine; Exploratory Behavior; Gastrodia; Glucosides; Homovanillic Acid; Humans; Hydroxyindoleacetic Acid; Male; Neurons; Plant Extracts; Rats, Sprague-Dawley; Receptor, Serotonin, 5-HT1A; Serotonin |
Appears in Collections: | 食品科技研究所 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.