https://scholars.lib.ntu.edu.tw/handle/123456789/413590
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Yang J.-H. | en_US |
dc.contributor.author | Chou C.-C. | en_US |
dc.contributor.author | Cheng Y.-W. | en_US |
dc.contributor.author | Sheen L.-Y. | en_US |
dc.contributor.author | Chou M.-C. | en_US |
dc.contributor.author | Yu H.-S. | en_US |
dc.contributor.author | Wei Y.-H. | en_US |
dc.contributor.author | Chung J.-G. | en_US |
dc.creator | Chung J.-G.;Wei Y.-H.;Yu H.-S.;Chou M.-C.;Sheen L.-Y.;Cheng Y.-W.;Chou C.-C.;Yang J.-H. | - |
dc.date.accessioned | 2019-07-11T09:16:47Z | - |
dc.date.available | 2019-07-11T09:16:47Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 2786915 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/413590 | - |
dc.description.abstract | Apoptosis is a particular process that leads to the programmed cell death, and it has been a potentially therapeutic target of cancer. In this study, we evaluated the possible apoptotic effects of glycolic acid on human leukemia cell line (HL-60) in vitro. The morphological changes, cell viability, apoptosis induction, and caspase-3 activity were measured by phase microscopy, flow cytometry, and Western blot analysis. Morphological changes including shrinkage of cells were clearly demonstrated in HL-60 cells treated with increasing concentrations of glycolic acid. Cell viability was significantly affected by glycolic acid treatment in a dose- and time-dependent manner. In comparison to the control group, glycolic acid treatment had a profound effect in the induction of apoptosis by flow cytometric assays. In the cell cycle analysis, glycolic acid caused the increased percentage of cells in G2/M phase and the decreased expression of the cyclin A and cyclin B1, suggesting the induction of G2/M arrest of cell cycle by glycolic acid. Moreover, glycolic acid treatment promoted caspase-9 and -3 activity in a dose-dependent manner, but caspse-8 activity was not affected during the same process. Glycolic acid co-administrated with broad-spectrum caspase inhibitor, z-VAD-fmk, caspase-3 activity was blunted and apoptosis was also markedly blocked in HL-60 cells. In conclusion, glycolic acid-induced apoptosis in HL-60 cells may be through the activation of caspase-3. Future studies focusing on cell signaling and biological significance of glycolic acid-induced apoptosis would lead to exploring the mechanisms of chemotherapeutic potency of glycolic acid in human cancers. ? 2004 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.relation.ispartof | Food and Chemical Toxicology | - |
dc.subject | Apoptosis | - |
dc.subject | Flow cytometry | - |
dc.subject | Glycolic acid | - |
dc.subject | Human leukemia HL-60 cells | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | antineoplastic agent; benzyloxycarbonylvalylalanylaspartyl fluoromethyl ketone; caspase 3; caspase 8; caspase 9; caspase inhibitor; cyclin A; cyclin B; glycolic acid; antigen expression; apoptosis; article; cancer; cell cycle G2 phase; cell strain HL 60; cell structure; cell viability; concentration response; controlled study; drug effect; drug potency; drug screening; enzyme activation; enzyme activity; flow cytometry; human; human cell; microscopy; signal transduction; Western blotting; Amino Acid Chloromethyl Ketones; Analysis of Variance; Apoptosis; Blotting, Western; Caspase 3; Caspase 9; Caspases; Cell Cycle; Cell Survival; Cyclins; Cysteine Proteinase Inhibitors; DNA; Dose-Response Relationship, Drug; Enzyme Activation; Flow Cytometry; Glycolates; HL-60 Cells; Humans; Indicators and Reagents; Time Factors | - |
dc.title | Effects of glycolic acid on the induction of apoptosis via caspase-3 activation in human leukemia cell line (HL-60) | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.fct.2004.07.004 | - |
dc.identifier.scopus | 2-s2.0-4444340999 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-4444340999&doi=10.1016%2fj.fct.2004.07.004&partnerID=40&md5=8e59a59f23d8fe54eee6514ef1e360ce | - |
dc.relation.pages | 1777-1784 | - |
dc.relation.journalvolume | 42 | - |
dc.relation.journalissue | 11 | - |
item.fulltext | no fulltext | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Food Science and Technology | - |
crisitem.author.orcid | 0000-0001-5541-652X | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
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