https://scholars.lib.ntu.edu.tw/handle/123456789/413595
Title: | Effect of garlic active principle, diallyl disulfide, on cell viability, lipid peroxidation, glutathione concentration and its related enzyme activities in primary rat hepatocytes | Authors: | Sheen L.-Y. Sheu S.-F. Tsai S.-J. Meng R.H.C. Lii C.-K. |
Issue Date: | 1999 | Journal Volume: | 27 | Journal Issue: | 1 | Start page/Pages: | 95-105 | Source: | American Journal of Chinese Medicine | Abstract: | This study investigated the effects of various concentrations and incubation time intervals of diallyl disulfide (DADS), the active principle of garlic, on: 1. cell viability, 2. lipid peroxidation. and 3. glutathione (GSH) concentration and its related enzyme activities of rat hepatocytes. According to the results of LDH leakage and microscopic examination, 0.5 and 1 mM DADS did not significantly affect the viability of hepatocytes. However, significant decrease in cell viability according to increased LDH leakage and significant changes in morphology of hepatocytes were observed at 2 mM DADS (P<0.05). Lipid peroxidation was also detected when the hepatocytes were treated with 2 mM DADS. At 0.5 mM DADS, a higher GSH content was found in the hepatocytes although not at a statistically significant level. 0.5 and 1 mM DADS has little effect on the activities of glutathione-Stransferase (GST) and glutathione peroxidase (GPx); however a significant decrease in GST, GPx and glutathione reductase (GRd) activities was observed at 2 mM DADS. Once the media of 2 mM DADS was replaced with fresh medium at 24 hr treatment, the activities of GST, GRd and GPx were recovered, although they were still lower than the control values. ? 1999 Institute for Advanced Research in Asian Scsience and Medicine. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413595 | ISSN: | 0192415X | DOI: | 10.1142/S0192415X99000124 |
Appears in Collections: | 食品科技研究所 |
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