https://scholars.lib.ntu.edu.tw/handle/123456789/413597
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Wang S.-T. | en_US |
dc.contributor.author | Chen H.-W. | en_US |
dc.contributor.author | Sheen L.-Y. | en_US |
dc.contributor.author | Lii C.-K. | en_US |
dc.creator | Lii C.-K.;Sheen L.-Y.;Chen H.-W.;Wang S.-T. | - |
dc.date.accessioned | 2019-07-11T09:16:48Z | - |
dc.date.available | 2019-07-11T09:16:48Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 223166 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/413597 | - |
dc.description.abstract | Methionine and cysteine are constituents of glutathione. To understand the effects of these two sulfur amino acids on the glutathione (GSH)- dependent detoxification defense system, intracellular GSH and GSH-related enzyme activities, including GSH peroxidase, GSH reductase, GSH S-transferase (GST) and £^-glutamylcysteine synthetase, were determined. In addition, the expression of three GST isozymes and carbonic anhydrase III (CA III) was examined. Hepatocytes isolated from male Sprague-Dawley rats were cultured with 0.1, 0.3, 0.5 or 1.0 mmol/L each of L-methionine and L-cysteine, for up to 7 d. Cells incubated with 0.5 or 1.0 mmol/L methionine and cysteine had increased intracellular GSH. A twofold increase was observed on d 6 compared with freshly isolated hepatocytes (P < 0.05). However, intracellular GSH was lower in cells treated with 0.3 or 0.1 mmol/L each of methionine and cysteine than in cells tested with 0.5 or 1.0 mmol/L. Although the GSH level differed significantly between cells cultured with 0.3 or 1.0 mmol/L of methionine and cysteine, GSH-related enzymes did not differ at these two concentrations. The activity generally remained constant for the first 24 h, then increased up to d 4. Immunodetection analysis revealed no difference in the level of CA III and GST isoforms, Ya, Yb and Yp, with amino acids each at a concentration of at least 0.3 mmol/L. Yp expression steadily increased up to d 7. Most proteins decreased rapidly after 48 h when cultured with 0.1 mmol/L of methionine and cysteine; however, the Yp level increased up to d 6. In conclusion, results indicate that a twofold increase of intracellular GSH is reached by adding methionine and cysteine at a concentration >0.5 mmol/L to the culture medium. The concentrations of methionine and cysteine for maintaining hepatic GSH are higher than for GSH-related enzyme activity and for GST isoform expression. | - |
dc.language | English | - |
dc.relation.ispartof | Journal of Nutrition | - |
dc.subject | Glutathione | - |
dc.subject | Glutathione S-transferase isozymes | - |
dc.subject | Hepatocytes | - |
dc.subject | Rats | - |
dc.subject | Sulfur amino acids | - |
dc.title | Methionine and cysteine affect glutathione level, glutathione-related enzyme activities and the expression of glutathione S-transferase isozymes in rat hepatocytes | en_US |
dc.type | journal article | en |
dc.identifier.scopus | 2-s2.0-0030830939 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030830939&partnerID=40&md5=e581301212304620211e0e739faa1e13 | - |
dc.relation.pages | 2135-2141 | - |
dc.relation.journalvolume | 127 | - |
dc.relation.journalissue | 11 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Food Science and Technology | - |
crisitem.author.orcid | 0000-0001-5541-652X | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
顯示於: | 食品科技研究所 |
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