https://scholars.lib.ntu.edu.tw/handle/123456789/413632
標題: | Induction of apoptosis by hydroxydibenzoylmethane through coordinative modulation of cyclin D3, Bcl-X L , and Bax, release of cytochrome c, and sequential activation of caspases in human colorectal carcinoma cells | 作者: | MIN-HSIUNG PAN Huang M.-C. Wang Y.-J. Lin J.-K. Lin, Chao-Hsien |
關鍵字: | Apoptosis;Caspase-2;Caspase-3;Caspase-9;Caspase-activated deoxyribonuclease;Cytochrome c;DBM;DNA fragmentation factor;HDB;HMDB;Poly-(ADP-ribose) polymerase | 公開日期: | 2003 | 卷: | 51 | 期: | 14 | 起(迄)頁: | 3977-3984 | 來源出版物: | Journal of Agricultural and Food Chemistry | 摘要: | DBM (dibenzoylmethane) is a minor constituent of licorice that has antimutagenic activity. However, its other biological activities are not well-known. The structurally related £]-diketones hydroxydibenzoylmethane (HDB) and hydroxymethyldibenzoylmethane (HMDB) were able to induce apoptosis in colorectal carcinoma COLO 205 cells. Thus, the effect of structurally related £]-diketones on cell viability, DNA fragmentation, and caspase activity was assessed. The potency of these compounds on these features of apoptosis were in the order of HDB > HMDB > DBM in colorectal carcinoma COLO 205 cells. Here, we found that HDB-induced apoptotic cell death was accompanied by upregulation of cyclin D3, Bax, and p21 and down-regulation of Bcl-X L , while HDB had no effect on the levels of Bcl-2 and Bad protein. These results indicate that HDB allows caspase-activated deoxyribonuclease to enter the nucleus and degrade chromosomal DNA and induces DFF-45 degradation. It is suggested that HDB-induced apoptosis is triggered by the release of cytochrome c into cytosol, procaspase-9 processing, activation of caspase-3 and caspase-2, degradation of PARP, and DNA fragmentation caused by the caspase-activated deoxyribonuclease through the digestion of DFF-45. The induction of apoptosis by HDB may provide a pivotal mechanism for its cancer chemopreventive action. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413632 | ISSN: | 00218561 | DOI: | 10.1021/jf034094i | SDG/關鍵字: | antimutagenic agent; caspase; caspase 2; caspase 3; cyclin D3; cytochrome c; deoxyribonuclease; diketone; enzyme inhibitor; enzyme precursor; hydroxydibenzoylmethane; hydroxymethyldibenzoylmethane; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; protein BAD; protein Bax; protein bcl 2; protein bcl xl; protein p21; unclassified drug; apoptosis; article; cell line; cell strain COLO 205; cell viability; chemoprophylaxis; colorectal carcinoma; controlled study; DNA degradation; DNA strand breakage; drug mechanism; drug potency; drug structure; enzyme activation; enzyme release; gene expression; Glycyrrhiza; human; human cell; protein degradation; signal transduction; Antioxidants; Apoptosis; bcl-2-Associated X Protein; bcl-X Protein; Benzoates; Caspase 3; Caspases; Cell Survival; Chalcones; Colorectal Neoplasms; Cyclins; Cytochrome c Group; DNA Fragmentation; Dose-Response Relationship, Drug; Enzyme Activation; Enzyme Inhibitors; Gene Expression; Humans; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-bcl-2; Tumor Cells, Cultured; Glycyrrhiza |
顯示於: | 食品科技研究所 |
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