https://scholars.lib.ntu.edu.tw/handle/123456789/413663| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Liu J. | en_US |
| dc.contributor.author | Zhou R. | en_US |
| dc.contributor.author | Li L. | en_US |
| dc.contributor.author | Peters B.M. | en_US |
| dc.contributor.author | Li B. | en_US |
| dc.contributor.author | Lin C.-W. | en_US |
| dc.contributor.author | Chuang T.-L. | en_US |
| dc.contributor.author | Chen D. | en_US |
| dc.contributor.author | Zhao X. | en_US |
| dc.contributor.author | Xiong Z. | en_US |
| dc.contributor.author | Xu Z. | en_US |
| dc.contributor.author | Shirtliff M.E. | en_US |
| dc.creator | Liu J.;Zhou R.;Li L.;Peters B.M.;Li B.;Lin C.-W.;Chuang T.-L.;Chen D.;Zhao X.;Xiong Z.;Xu Z.;Shirtliff M.E. | - |
| dc.date.accessioned | 2019-07-15T04:23:59Z | - |
| dc.date.available | 2019-07-15T04:23:59Z | - |
| dc.date.issued | 2017 | - |
| dc.identifier.issn | 9232508 | - |
| dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85007425406&doi=10.1016%2fj.resmic.2016.11.002&partnerID=40&md5=60e46e425731732dd03894fc6db8403b | - |
| dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/413663 | - |
| dc.description.abstract | As major food-borne pathogens worldwide, Escherichia coli are capable of toxin production directly causing severe human disease. However, routine methods are incapable of detecting viable but non-culturable (VBNC) bacteria in food products and raw materials, leading to false-negative identification. In this study, VBNC E. coli O157 strains were acquired after cryopreservation at ?20?°C, with and without freeze-thawing; morphology was observed to be of shorter rod-shape, and toxin expression remained at relatively high levels. PMA-PCR assay for VBNC detection was also validated. Therefore, these results suggest that VBNC E. coli O157 strains may represent a strong threat to public health and food safety. ? 2016 Institut Pasteur | - |
| dc.publisher | Elsevier Masson SAS | - |
| dc.relation.ispartof | Research in Microbiology | - |
| dc.subject | Cryopreservation; E. coli O157; PMA-PCR; Toxin expression; VBNC state | - |
| dc.subject.other | Shiga toxin; bacterial toxin; Article; bacterial strain; controlled study; cryopreservation; enterohemorrhagic Escherichia coli; gene expression; nonhuman; polymerase chain reaction; priority journal; cytology; Escherichia coli O157; food safety; genetics; human; isolation and purification; microbial viability; physiology; scanning electron microscopy; Bacterial Toxins; Cryopreservation; Escherichia coli O157; Food Safety; Humans; Microbial Viability; Microscopy, Electron, Scanning; Polymerase Chain Reaction | - |
| dc.subject.other | [SDGs]SDG3 | - |
| dc.title | Viable but non-culturable state and toxin gene expression of enterohemorrhagic Escherichia coli O157 under cryopreservation | en_US |
| dc.type | journal article | - |
| dc.identifier.doi | 10.1016/j.resmic.2016.11.002 | - |
| dc.identifier.pmid | 27884785 | - |
| dc.identifier.scopus | 2-s2.0-85007425406 | - |
| dc.relation.pages | 188-193 | - |
| dc.relation.journalvolume | 168 | - |
| dc.relation.journalissue | 3 | - |
| item.cerifentitytype | Publications | - |
| item.openairetype | journal article | - |
| item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
| item.fulltext | no fulltext | - |
| item.grantfulltext | none | - |
| crisitem.author.dept | Biomedical Electronics and Bioinformatics | - |
| crisitem.author.dept | Applied Mechanics | - |
| crisitem.author.dept | Biomedical Engineering | - |
| crisitem.author.dept | Electrical Engineering | - |
| crisitem.author.orcid | 0000-0002-8721-3441 | - |
| crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
| crisitem.author.parentorg | College of Engineering | - |
| crisitem.author.parentorg | College of Medicine | - |
| crisitem.author.parentorg | College of Engineering | - |
| crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
| Appears in Collections: | 醫學工程學研究所 | |
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