https://scholars.lib.ntu.edu.tw/handle/123456789/413735
標題: | High-sensitivity detection of carbohydrate antigen 15-3 using a gold/zinc oxide thin film surface plasmon resonance-based biosensor | 作者: | Chang C.-C. Chiu N.-F. Lin D.S. Chu-Su Y. Liang Y.-H. Lin C.-W. |
公開日期: | 2010 | 卷: | 82 | 期: | 4 | 起(迄)頁: | 1207-1212 | 來源出版物: | Analytical Chemistry | 摘要: | We report that gold/zinc oxide (Au/ZnO) nanocomposite films were effectively employed to enhance the performance of surface plasmon resonance (SPR) for the detection of tumor markers. Carbohydrate antigen 15.3 (CA15-3), a tumor marker for breast cancer, was chosen as a model analyte. We analyzed intensity response to the samples at various concentrations (0.0125 U/mL to 160 U/mL) in pleural fluid to evaluate the detection capability of the SPR biosensor based on Au/ZnO thin films. The linear range extended from 1 to 40 U/mL with a correlation coefficient of R< sup> 2< /sup> = 0.991 and a limit of detection reaching 0.025 U/mL at a signal-to-noise ratio of 3:1. Compared with the degree of the shift in SPR intensity induced by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers was increased by at least 2 fold over that on the gold/chromium (Au/Cr) layers. In addition, we determined that the Au/ZnO layers allowed for a detection limit 4 times lower than the Au/Cr layers, which are in widespread use as the sensing interfaces in current SPR-based detectors. In conclusion, the use of Au/ ZnO films greatly enhanced the SPR signal yield for this bimolecular interaction and showed high sensitivity. ? 2010 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-76849090412&doi=10.1021%2fac901797j&partnerID=40&md5=7c3001e88ec2b789bf7bb98e7d3ff6ea https://scholars.lib.ntu.edu.tw/handle/123456789/413735 |
ISSN: | 32700 | DOI: | 10.1021/ac901797j | SDG/關鍵字: | Analytes; Bimolecular interactions; Breast Cancer; Correlation coefficient; Detection capability; Detection limits; High sensitivity; High-sensitivity detection; Limit of detection; Linear range; Nanocomposite film; Oxide thin films; Specific binding; SPR biosensor; SPR signals; Tumor markers; ZnO films; Antigens; Biosensors; Carbohydrates; Detectors; Nanocomposites; Oxide films; Plasmons; Signal detection; Signal to noise ratio; Thin films; Tumors; Zinc oxide; Surface plasmon resonance; antibody; chromium; gold; tumor antigen; zinc oxide; animal; article; chemistry; human; immunoassay; immunology; methodology; pleura cavity; surface plasmon resonance; surface property; Animals; Antibodies; Antigens, Tumor-Associated, Carbohydrate; Chromium; Gold; Humans; Immunoassay; Pleural Cavity; Surface Plasmon Resonance; Surface Properties; Zinc Oxide |
顯示於: | 醫學工程學研究所 |
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