DC 欄位 | 值 | 語言 |
dc.contributor.author | Tung C.-H. | en_US |
dc.contributor.author | Lin C.-C. | en_US |
dc.contributor.author | Wang H.-J. | en_US |
dc.contributor.author | Chen S.-F. | en_US |
dc.contributor.author | Sheu F. | en_US |
dc.contributor.author | FUU SHEU | en_US |
dc.contributor.author | HUEI WANG | en_US |
dc.creator | Lu T.-J.;Sheu F.;Chen S.-F.;Wang H.-J.;Lin C.-C.;Tung C.-H. | - |
dc.date.accessioned | 2019-07-16T08:48:48Z | - |
dc.date.available | 2019-07-16T08:48:48Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 10219498 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/414023 | - |
dc.description.abstract | Fungal immunomodulatory protein (FIP-fve) is a potential functional food ingredient. However, undesirable component flammutoxin (FTX) would occur in the extracted fraction of FIP-fve. In this paper, an application of heating processing instead of the intensive separation process was employed in fractionation of FIP-fve, meanwhile, exclusion of FTX was reached. Contents of FIP-fve and FTX were monitored by HPLC-UV-ESI-MS. Both FIP-fve and FTX had higher thermal stability in a lower concentration solution. Cold water could effectively extract FIP-fve and FTX from fresh mushroom without acetic acid and disulfide-bond breaking agent £]-mercaptoethanol commonly used in biochemical studies. Heating cold water extract contained 580 £gg/mL FIP-fve and 452 £gg/mL FTX at 60 ¢XC for 5 min could effectively exclude FTX and remain 75% of FIP-fve. Adding 0.1 M trehalose or 20% ethanol did not significantly alter the stability of both proteins. The method developed is an applicable procedure for preparing FIP-fve solution free of FTX. ? 2018 | en_US |
dc.language | English | - |
dc.relation.ispartof | Journal of Food and Drug Analysis | en_US |
dc.subject | FIP-fve | en_US |
dc.subject | Flammulina velutipes | en_US |
dc.subject | Flammutoxin | en_US |
dc.subject | HPLC-UV-ESI-MS | en_US |
dc.subject | Protein stability | en_US |
dc.title | Application of thermal stability difference to remove flammutoxin in fungal immunomodulatory protein, FIP-fve, extract from Flammulina velutipes | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.jfda.2017.12.010 | - |
dc.identifier.doi | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040962424&doi=10.1016%2fj.jfda.2017.12.010&partnerID=40&md5=d771a95ca0a36e03ab1732cc07912e93 | - |
dc.identifier.scopus | 2-s2.0-85040962424 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040962424&doi=10.1016%2fj.jfda.2017.12.010&partnerID=40&md5=d771a95ca0a36e03ab1732cc07912e93 | - |
dc.relation.pages | 1005-1014 | en_US |
dc.relation.journalvolume | 26 | en_US |
dc.relation.journalissue | 3 | en_US |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | with fulltext | - |
crisitem.author.dept | Horticulture and Landscape Architecture | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Communication Engineering | - |
crisitem.author.dept | Electrical Engineering | - |
crisitem.author.orcid | 0000-0001-9794-3483 | - |
crisitem.author.orcid | 0000-0002-9903-1979 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
顯示於: | 食品科技研究所
|