|Title:||Pilot scale repeated fed-batch fermentation processes of the wine yeast Dekkera bruxellensis for mass production of resveratrol from Polygonum cuspidatum||Authors:||Kuo H.-P.
|Keywords:||Dekkera bruxellensis;Polygonum cuspidatum;Repeated fed-batch bioconversion;Resveratrol;£]-Glucosidase||Issue Date:||2017||Journal Volume:||243||Start page/Pages:||986-993||Source:||Bioresource Technology||Abstract:||
Resveratrol has long been used as an ingredient in functional foods. Currently, Polygonum cuspidatum extract is the greatest natural source for resveratrol because of high concentrations of glycosidic-linked resveratrol. Thus, developing a cost-effective procedure to hydrolyze glucoside could substantially enhance resveratrol production from P. cuspidatum. This study selected Dekkera bruxellensis from several microorganisms based on its bioconversion and enzyme-specific activities. We demonstrated that the cells could be reused at least nine times while maintaining an average of 180.67?U/L £]-glucosidase activity. The average resveratrol bioconversion efficiency within five rounds of repeated usage was 108.77?¡Ó?0.88%. This process worked effectively when the volume was increased to 1200?L, a volume at which approximately 35?mg?L?1?h?1 resveratrol per round was produced. This repeated fed-batch bioconversion process for resveratrol production is comparable to enzyme or cell immobilization strategies in terms of reusing cycles, but without incurring additional costs for immobilization. ? 2017 Elsevier Ltd
|Appears in Collections:||食品科技研究所|
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