https://scholars.lib.ntu.edu.tw/handle/123456789/414324
標題: | Modification of flower colour by suppressing β-ring carotene hydroxylase genes in Oncidium | 作者: | Wang, H. M. To, K. Y. HSI-MEI LAI SHIH-TONG JENG |
關鍵字: | HPLC | Lutein | Neoxanthin | Real-time PCR | Transgenic plants | Violaxanthin | 公開日期: | 1-一月-2016 | 出版社: | WILEY-BLACKWELL | 卷: | 18 | 期: | 2 | 起(迄)頁: | 220 | 來源出版物: | Plant Biology | 摘要: | © 2016 German Botanical Society and Royal Botanical Society of the Netherlands. Oncidium 'Gower Ramsey' (Onc. GR) is a popular cut flower, but its colour is limited to bright yellow. The β-ring carotene hydroxylase (BCH2) gene is involved in carotenoid biogenesis for pigment formation. However, the role of BCH2 in Onc. GR is poorly understood. Here, we investigated the functions of three BCH2 genes, BCH-A2, BCH-B2 and BCH-C2 isolated from Onc. GR, to analyse their roles in flower colour. RT-PCR expression profiling suggested that BCH2 was mainly expressed in flowers. The expression of BCH-B2 remained constant while that of BCH-A2 gradually decreased during flower development. Using Agrobacterium tumefaciens to introduce BCH2 RNA interference (RNAi), we created transgenic Oncidium plants with down-regulated BCH expression. In the transgenic plants, flower colour changed from the bright yellow of the wild type to light and white-yellow. BCH-A2 and BCH-B2 expression levels were significantly reduced in the transgenic flower lips, which make up the major portion of the Oncidium flower. Sectional magnification of the flower lip showed that the amount of pigmentation in the papillate cells of the adaxial epidermis was proportional to the intensity of yellow colouration. HPLC analyses of the carotenoid composition of the transgenic flowers suggested major reductions in neoxanthin and violaxanthin. In conclusion, BCH2 expression regulated the accumulation of yellow pigments in the Oncidium flower, and the down-regulation of BCH-A2 and BCH-B2 changed the flower colour from bright yellow to light and white-yellow. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414324 | ISSN: | 14358603 | DOI: | 10.1111/plb.12399 |
顯示於: | 植物科學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。