https://scholars.lib.ntu.edu.tw/handle/123456789/414330
標題: | Identification of plant vacuolar transporters mediating phosphate storage | 作者: | Liu, Tzu Yin Huang, Teng Kuei SHU-YI YANG Hong, Yu Ting Huang, Sheng Min Wang, Fu Nien Chiang, Su Fen Tsai, Shang Yueh Lu, Wen Chien Chiou, Tzyy Jen |
公開日期: | 31-三月-2016 | 出版社: | NATURE PUBLISHING GROUP | 卷: | 7 | 期: | 1 | 來源出版物: | Nature Communications | 摘要: | Plant vacuoles serve as the primary intracellular compartments for inorganic phosphate (Pi) storage. Passage of Pi across vacuolar membranes plays a critical role in buffering the cytoplasmic Pi level against fluctuations of external Pi and metabolic activities. Here we demonstrate that the SPX-MFS proteins, designated as PHOSPHATE TRANSPORTER 5 family (PHT5), also named Vacuolar Phosphate Transporter (VPT), function as vacuolar Pi transporters. Based on 31 P-magnetic resonance spectroscopy analysis, Arabidopsis pht5;1 loss-of-function mutants accumulate less Pi and exhibit a lower vacuolar-to-cytoplasmic Pi ratio than controls. Conversely, overexpression of PHT5 leads to massive Pi sequestration into vacuoles and altered regulation of Pi starvation-responsive genes. Furthermore, we show that heterologous expression of the rice homologue OsSPX-MFS1 mediates Pi influx to yeast vacuoles. Our findings show that a group of Pi transporters in vacuolar membranes regulate cytoplasmic Pi homeostasis and are required for fitness and plant growth. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414330 | ISSN: | 2041-1723 | DOI: | https://api.elsevier.com/content/abstract/scopus_id/84962782977 10.1038/ncomms11095 |
顯示於: | 植物科學研究所 |
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ncomms11095 (1).pdf | 1.24 MB | Adobe PDF | 檢視/開啟 |
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