https://scholars.lib.ntu.edu.tw/handle/123456789/414346
Title: | PECTIN METHYLESTERASE34 contributes to heat tolerance through its role in promoting stomatal movement | Authors: | Huang, Ya Chen Wu, Hui Chen Wang, Yin Da Liu, Chia Hung Lin, Ching Chih Luo, Dan Li TSUNG-LUO JINN |
Issue Date: | 1-Jun-2017 | Publisher: | AMER SOC PLANT BIOLOGISTS | Journal Volume: | 174 | Journal Issue: | 2 | Start page/Pages: | 748 | Source: | Plant Physiology | Abstract: | © 2017 American Society of Plant Biologists. All Rights Reserved. Pectin, a major component of the primary cell wall, is synthesized in the Golgi apparatus and exported to the cell wall in a highly methylesterified form, then is partially demethylesterified by pectin methylesterases (PMEs; EC 3.1.1.11). PME activity on the status of pectin methylesterification profoundly affects the properties of pectin and, thereby, is critical for plant development and the plant defense response, although the roles of PMEs under heat stress (HS) are poorly understood. Functional genome annotation predicts that at least 66 potential PME genes are contained in Arabidopsis (Arabidopsis thaliana). Thermotolerance assays of PME gene T-DNA insertion lines revealed two null mutant alleles of PME34 (At3g49220) that both consistently showed reduced thermotolerance. Nevertheless, their impairment was independently associated with the expression of HS-responsive genes. It was also observed that PME34 transcription was induced by abscisic acid and highly expressed in guard cells. We showed that the PME34 mutation has a defect in the control of stomatal movement and greatly altered PME and polygalacturonase (EC 3.2.1.15) activity, resulting in a heat-sensitive phenotype. PME34 has a role in the regulation of transpiration through the control of the stomatal aperture due to its cell wall-modifying enzyme activity during the HS response. Hence, PME34 is required for regulating guard cell wall flexibility to mediate the heat response in Arabidopsis. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020468750&doi=10.1104%2fpp.17.00335&partnerID=40&md5=985b1bc92099a32aeb56c6d2ee9d6308 https://scholars.lib.ntu.edu.tw/handle/123456789/414346 |
ISSN: | 00320889 | DOI: | https://api.elsevier.com/content/abstract/scopus_id/85020468750 10.1104/pp.17.00335 |
SDG/Keyword: | abscisic acid; Arabidopsis protein; carboxylesterase; pectinesterase; Arabidopsis; cell membrane; cell wall; drug effects; evapotranspiration; gene expression regulation; genetics; heat shock response; metabolism; mutation; physiology; plant stoma; transgenic plant; Abscisic Acid; Arabidopsis; Arabidopsis Proteins; Carboxylic Ester Hydrolases; Cell Membrane; Cell Wall; Gene Expression Regulation, Plant; Heat-Shock Response; Mutation; Plant Stomata; Plant Transpiration; Plants, Genetically Modified |
Appears in Collections: | 植物科學研究所 |
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