https://scholars.lib.ntu.edu.tw/handle/123456789/414357
標題: | A class of soybean low molecular weight heat shock proteins: Immunological study and quantitation | 作者: | Hsieh, Ming Hsiun Chen, Ju Tzen TSUNG-LUO JINN Chen, Yih Ming Lin, Chu Yung |
公開日期: | 1-一月-1992 | 卷: | 99 | 期: | 4 | 起(迄)頁: | 1279 | 來源出版物: | Plant Physiology | 摘要: | Two major polypeptides of the 15- to 18-kilodalton class of soybean (Glycine max) heat shock proteins (HSPs), obtained from an HSP-enriched (NH4)2SO4 fraction separated by two-dimensional polyacrylamide gel electrophoresis, were used individually as antigens to prepare antibodies. Each of these antibody preparations reacted with its antigen and cross-reacted with 12 other 15- to 18-kilodalton HSPs. With these antibodies, the accumulation of the 15- to 18-kilodalton HSPs under various heat shock (HS) conditions was quantified. The 15- to 18-kilodalton HSPs began to be detectable at 35°C, and after 4 hours at 40° C they had accumulated to a maximum level of 1.54 micrograms per 100 micrograms of total protein in soybean seedlings and remained almost unchanged up to 24 hours after HS. Accumulation of the HSPs was reduced at temperatures higher than 40° C. At 42.5° C the HSPs were reduced to 1.02 micrograms per 100 micrograms, and at 45° C they were hardly detectable. A brief HS at 45° C (10 minutes), followed by incubation at 28° C, which also induced HSP synthesis, resulted in synthesis of this class of HSPs at levels up to 1.06 micrograms per 100 micrograms of total protein. Taking into consideration the previous data concerning the acquisition of thermotolerance in soybean seedlings, our estimation indicates that the accumulation of the 15- to 18-kilodalton HSPs to 0.76 to 0.98% of total protein correlated well with the establishment of thermotolerance. Of course, other HSPs, in addition to this group of proteins, may be required for the developement of thermotolerance. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414357 | ISSN: | 00320889 | DOI: | https://api.elsevier.com/content/abstract/scopus_id/0000023437 10.1104/pp.99.4.1279 |
顯示於: | 植物科學研究所 |
檔案 | 描述 | 大小 | 格式 | |
---|---|---|---|---|
1279.full.pdf | 1.46 MB | Adobe PDF | 檢視/開啟 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。