https://scholars.lib.ntu.edu.tw/handle/123456789/414385
標題: | Signal transduction and regulation of IbpreproHypSys in sweet potato | 作者: | Li, Yu Chi Wan, Wei Lin Lin, Jeng Shane Kuo, Yun Wei King, Yu Chi Chen, Yuchi SHIH-TONG JENG |
關鍵字: | hormones | insect interaction | mechanical | mechanical wounding response | oxidative stress | signalling | 公開日期: | 1-七月-2016 | 出版社: | WILEY-BLACKWELL | 卷: | 39 | 期: | 7 | 起(迄)頁: | 1576 | 來源出版物: | Plant Cell and Environment | 摘要: | © 2016 John Wiley & Sons Ltd Hydroxyproline-rich glycopeptides (HypSys) are small signalling peptides containing 18–20 amino acids. The expression of IbpreproHypSys, encoding the precursor of IbHypSys, was induced in sweet potato (Ipomoea batatas cv. Tainung 57) through wounding and IbHypSys treatments by using jasmonate and H2O2. Transgenic sweet potatoes overexpressing (OE) and silencing [RNA interference (RNAi)] IbpreproHypSys were created. The expression of the wound-inducible gene for ipomoelin (IPO) in the local and systemic leaves of OE plants was stronger than the expression in wild-type (WT) and RNAi plants after wounding. Furthermore, grafting experiments indicated that IPO expression was considerably higher in WT stocks receiving wounding signals from OE than from RNAi scions. However, wounding WT scions highly induced IPO expression in OE stocks. These results indicated that IbpreproHypSys expression contributed towards sending and receiving the systemic signals that induced IPO expression. Analysing the genes involved in the phenylpropanoid pathway demonstrated that lignin biosynthesis was activated after synthetic IbHypSys treatment. IbpreproHypSys expression in sweet potato suppressed Spodoptera litura growth. In conclusion, wounding induced the expression of IbpreproHypSys, whose protein product was processed into IbHypSys. IbHypSys stimulated IbpreproHypSys and IPO expression and enhanced lignin biosynthesis, thus protecting plants from insects. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966271176&doi=10.1111%2fpce.12729&partnerID=40&md5=ace1b0205995781f4538855c6e7224f6 https://scholars.lib.ntu.edu.tw/handle/123456789/414385 |
ISSN: | 01407791 | DOI: | https://api.elsevier.com/content/abstract/scopus_id/84966271176 10.1111/pce.12729 |
顯示於: | 植物科學研究所 |
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