https://scholars.lib.ntu.edu.tw/handle/123456789/414669
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | C. K. Chen | en US |
dc.contributor.author | C. P. Yu | en US |
dc.contributor.author | S. C. Li | en US |
dc.contributor.author | S. M. Wu | en US |
dc.contributor.author | M. Y. J. Lu | en US |
dc.contributor.author | Y. H. Chen | en US |
dc.contributor.author | D. R. Chen | en US |
dc.contributor.author | C. S. Ng | en US |
dc.contributor.author | C. T. Ting | en US |
dc.contributor.author | W. H. Li | en US |
dc.creator | W. H. Li;C. T. Ting;C. S. Ng;D. R. Chen;Y. H. Chen;M. Y. J. Lu;S. M. Wu;S. C. Li;C. P. Yu;C. K. Chen | - |
dc.date.accessioned | 2019-07-24T01:40:41Z | - |
dc.date.available | 2019-07-24T01:40:41Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 14712164 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/414669 | - |
dc.description.abstract | Background: Long non-coding RNAs (lncRNAs) are important in various biological processes, but very few studies on lncRNA have been conducted in birds. To identify IncRNAs expressed during feather development, we analyzed single-stranded RNA-seq (ssRNA-seq) data from the anterior and posterior dorsal regions during zebra finch (Taeniopygia guttata) embryonic development. Using published transcriptomic data, we further analyzed the evolutionary conservation of IncRNAs in birds and amniotes. Results: A total of 1,081 lncRNAs, including 965 intergenic lncRNAs (lincRNAs), 59 intronic lncRNAs, and 57 antisense lncRNAs (lncNATs), were identified using our newly developed pipeline. These avian IncRNAs share similar characteristics with lncRNAs in mammals, such as shorter transcript length, lower exon number, lower average expression level and less sequence conservation than mRNAs. However, the proportion of lncRNAs overlapping with transposable elements in birds is much lower than that in mammals. We predicted the functions of IncRNAs based on the enriched functions of co-expressed protein-coding genes. Clusters of lncRNAs associated with natal down development were identified. The sequences and expression levels of candidate lncRNAs that shared conserved sequences among birds were validated by qPCR in both zebra finch and chicken. Finally, we identified three highly conserved lncRNAs that may be associated with natal down development. Conclusions: Our study provides the first systematical identification of avian lncRNAs using ssRNA-seq analysis and offers a resource of embryonically expressed lncRNAs in zebra finch. We also predicted the biological function of identified lncRNAs. | - |
dc.publisher | BioMed Central Ltd. | - |
dc.relation.ispartof | BMC Genomics | - |
dc.subject | Feather development; LncRNA; SsRNA-seq; Zebra finch | - |
dc.title | Identification and evolutionary analysis of long non-coding RNAs in zebra finch | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1186/s12864-017-3506-z | - |
dc.relation.pages | Article number 117 | - |
dc.relation.journalvolume | 18 | - |
dc.relation.journalissue | 1 | - |
item.fulltext | no fulltext | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Life Science | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Ecology and Evolutionary Biology | - |
crisitem.author.dept | Master Program in Global Agriculture Technology and Genomic Science (Global ATGS) | - |
crisitem.author.dept | Genome and Systems Biology Degree Program | - |
crisitem.author.dept | Master's Program in Biodiversity (MPB) | - |
crisitem.author.orcid | 0000-0003-1802-8864 | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | International College | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | International College | - |
顯示於: | 生命科學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。