https://scholars.lib.ntu.edu.tw/handle/123456789/415873
Title: | Polydopamine-coated gold nanostar for combined antitumor and antiangiogenic therapy in multidrug-resistant breast cancer | Authors: | You, Y.-H. Lin, Y.-F. Nirosha, B. Chang, H.-T. Huang, Y.-F. |
Keywords: | Antiangiogenesis; Cancer combination therapy; Drug delivery; Gold nanostar; Multidrug resistance; Polydopamine | Issue Date: | 2019 | Journal Volume: | 3 | Journal Issue: | 3 | Start page/Pages: | 266-283 | Source: | Nanotheranostics | Abstract: | Cancer combination therapy can improve treatment efficacy and is widely utilized in the biomedical field. In this paper, we propose a facile strategy to develop a polydopamine (PDA)-coated Au nanostar (NS@PPFA) as a multifunctional nanoplatform for cancer targeting and combination therapy. The Au nanostar demonstrated high photothermal conversion efficiency because of the tip-enhanced plasmonic effect. Modification of PDA and folic acid on the NS surface improved its drug-loading efficiency and targeting capability. In vitro, compared with nontargeted cells, targeted breast cancer MCF-7 cells demonstrated efficient uptake of chemodrug-loaded NS-D@PPFA through the receptor-mediated endocytosis pathway. In combination with the photothermal effect induced by near-infrared laser irradiation, controlled payload release could be activated in response to both internal (acid) and external (photothermal) stimuli, leading to an efficient chemo-photothermal action against MCF-7 cells and drug-resistant MCF-7/ADR cells. By contrast, cellular damage was less obvious in normal HaCaT (human skin keratinocytes) and NIH-3T3 cells (murine fibroblasts). In addition, payload-free NS@PPFA exhibited a high binding affinity (Kd = 2.68 × 10-10 M) toward vascular endothelial growth factor (VEGF-A165), which was at least two orders of magnitude stronger than that of highly abundant plasma proteins, such as human serum albumin. Furthermore, in vitro study showed that NS@PPFA could effectively inhibit VEGF-A165-induced proliferation, migration, and tube formation of human umbilical vein endothelial cells, resulting in additional therapeutic benefits for eradicating tumors through a simultaneous antiangiogenic action in chemo-photothermal treatment. The combined treatment also exhibited the lowest microvessel density, leading to a potent antitumor effect in vivo. Overall, our "all-in-one" nanoplatform is highly promising for tumor therapy, enabling effective treatment against multidrug-resistant cancers. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069262237&doi=10.7150%2fntno.36842&partnerID=40&md5=32b2a720eb644787ae6f1216d98e0ba9 https://scholars.lib.ntu.edu.tw/handle/123456789/415873 |
DOI: | 10.7150/ntno.36842 | SDG/Keyword: | angiogenesis inhibitor; antineoplastic agent; gold; indole derivative; nanoparticle; polydopamine; polymer; vasculotropin A; angiogenesis; animal; apoptosis; breast tumor; cell motion; cell proliferation; chemistry; drug effect; drug release; drug resistance; endocytosis; female; human; MCF-7 cell line; metabolism; mouse; multidrug resistance; NIH 3T3 cell line; nude mouse; phototherapy; temperature; thermotherapy; time factor; tissue distribution; ultrastructure; umbilical vein endothelial cell; Angiogenesis Inhibitors; Animals; Antineoplastic Agents; Apoptosis; Breast Neoplasms; Cell Movement; Cell Proliferation; Drug Liberation; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Endocytosis; Female; Gold; Human Umbilical Vein Endothelial Cells; Humans; Hyperthermia, Induced; Indoles; MCF-7 Cells; Mice; Mice, Nude; Nanoparticles; Neovascularization, Physiologic; NIH 3T3 Cells; Phototherapy; Polymers; Temperature; Time Factors; Tissue Distribution; Vascular Endothelial Growth Factor A |
Appears in Collections: | 化學系 |
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