https://scholars.lib.ntu.edu.tw/handle/123456789/416267
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lin, Bo-Shiou | en_US |
dc.contributor.author | Tsai, Pei-Yun | en_US |
dc.contributor.author | Hsieh, Wan-Yun | en_US |
dc.contributor.author | Tsao, Hsiao-Wei | en_US |
dc.contributor.author | Liu, Meng-Wei | en_US |
dc.contributor.author | Grenningloh, Roland | en_US |
dc.contributor.author | LI-FANG WANG | en_US |
dc.contributor.author | Ho, I-Cheng | en_US |
dc.contributor.author | SHI-CHUEN MIAW | en_US |
dc.creator | Lin, Bo-Shiou;Tsai, Pei-Yun;Hsieh, Wan-Yun;Tsao, Hsiao-Wei;Liu, Meng-Wei;Grenningloh, Roland;Li-Fang Wang;Ho, I-Cheng;Shi-Chuen Miaw | - |
dc.date.accessioned | 2019-08-06T05:10:42Z | - |
dc.date.available | 2019-08-06T05:10:42Z | - |
dc.date.issued | 2010-04 | - |
dc.identifier.issn | 0014-2980 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951068773&doi=10.1002%2feji.200939788&partnerID=40&md5=8b8de18bbc4045a511cde56564e3f141 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/416267 | - |
dc.description.abstract | The function of transcription factors can be critically regulated by SUMOylation. c-Maf, the cellular counterpart of v-maf oncogene, is a potent transactivator of the IL-4 gene in Th2 cells. We found in a yeast two-hybrid screen that c-Maf can interact with Ubc9 and PIAS1, two key enzymes of the SUMOylation pathway. In this study, we report that c-Maf co-localized with these two SUMO (small ubiquitin-like modifier) ligases in the nucleus and that c-Maf can be SUMOylated in vitro and also in primary Th2 cells. We also demonstrated that lysine-33 is the dominant, if not the only, SUMO acceptor site of c-Maf. SUMOylation of c-Maf attenuated its transcriptional activity. Reciprocally, a SUMOylation resistant c-Maf was more potent than WT-c-Maf in driving IL-4 production in c-Maf-deficient Th2 cells. Furthermore, we showed that ablation of the SUMO site did not alter the subcellular localization or the stability of c-Maf protein but instead enhanced its recruitment to the Il4-promoter. We conclude that SUMOylation at lysine-33 is a functionally critical post-translational modification event of c-Maf in Th cells. | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY | en_US |
dc.relation.ispartof | European journal of immunology | en_US |
dc.subject | c-Maf; Il4 Gene regulation; SUMOylation | en_US |
dc.title | SUMOylation attenuates c-Maf-dependent IL-4 expression | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1002/eji.200939788 | - |
dc.identifier.pmid | 20127678 | - |
dc.identifier.scopus | 2-s2.0-77951068773 | - |
dc.identifier.isi | WOS:000277210800027 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/77951068773 | - |
dc.relation.pages | 1174 | en_US |
dc.relation.journalvolume | 40 | en_US |
dc.relation.journalissue | 4 | en_US |
dc.relation.pageend | 1184 | en_US |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Immunology | - |
crisitem.author.orcid | 0000-0002-1709-9401 | - |
crisitem.author.orcid | 0000-0003-1347-9367 | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 免疫學研究所 |
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